High lead-tolerant mutant Bacillus tropicus AT31-1 from rhizosphere soil of Pu-erh and its remediation mechanism

IF 9.7 1区环境科学与生态学 Q1 AGRICULTURAL ENGINEERING

Bioresource Technology Pub Date : 2024-11-07 DOI:10.1016/j.biortech.2024.131751

Qiang Li , Wenxia Yuan , Xiujuan Deng , Yaping Chen , Limei Li , Lijiao Chen , Raoqiong Che , Wei Huang , Yamin Wu , Chun Wang , Zejun Wang , Miao Zhou , Zongpei Fan , Baijuan Wang

{"title":"High lead-tolerant mutant Bacillus tropicus AT31-1 from rhizosphere soil of Pu-erh and its remediation mechanism","authors":"Qiang Li , Wenxia Yuan , Xiujuan Deng , Yaping Chen , Limei Li , Lijiao Chen , Raoqiong Che , Wei Huang , Yamin Wu , Chun Wang , Zejun Wang , Miao Zhou , Zongpei Fan , Baijuan Wang","doi":"10.1016/j.biortech.2024.131751","DOIUrl":null,"url":null,"abstract":"<div><div>In this study, we successfully generated the mutant strain <em>Bacillus tropicus</em> AT31-1 from AT31 through atmospheric room-temperature plasma mutagenesis. This mutant strain AT31-1 demonstrated an impressive 48.6 % removal efficiency in 400 mg/L lead medium. Comparative genomic analysis showed that the mutant strain AT31-1 had three mutation sites, which affect the efflux RND transporter permease subunit, the response regulator transcription factor, and a gene with unknown function. The transcriptional analysis showed a notable upregulation in the expression of 283 genes in AT31-1 as lead concentrations increased from 0 to 200 mg/L and then to 400 mg/L, which include zinc-transporting ATPase, ferrous iron transport protein B, NADH dehydrogenase, and others. The Gene ontology function of the peptide metabolic process, along with the KEGG pathway of carbon metabolism were identified as closely linked to the extreme lead tolerance of AT31-1. This study presents novel insights into the lead tolerance mechanisms of bacteria.</div></div>","PeriodicalId":258,"journal":{"name":"Bioresource Technology","volume":"416 ","pages":"Article 131751"},"PeriodicalIF":9.7000,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioresource Technology","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S096085242401455X","RegionNum":1,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURAL ENGINEERING","Score":null,"Total":0}

引用次数: 0

Abstract

In this study, we successfully generated the mutant strain Bacillus tropicus AT31-1 from AT31 through atmospheric room-temperature plasma mutagenesis. This mutant strain AT31-1 demonstrated an impressive 48.6 % removal efficiency in 400 mg/L lead medium. Comparative genomic analysis showed that the mutant strain AT31-1 had three mutation sites, which affect the efflux RND transporter permease subunit, the response regulator transcription factor, and a gene with unknown function. The transcriptional analysis showed a notable upregulation in the expression of 283 genes in AT31-1 as lead concentrations increased from 0 to 200 mg/L and then to 400 mg/L, which include zinc-transporting ATPase, ferrous iron transport protein B, NADH dehydrogenase, and others. The Gene ontology function of the peptide metabolic process, along with the KEGG pathway of carbon metabolism were identified as closely linked to the extreme lead tolerance of AT31-1. This study presents novel insights into the lead tolerance mechanisms of bacteria.

Abstract Image

查看原文本刊更多论文

普洱根瘤土壤中的高耐铅突变体枯草芽孢杆菌 AT31-1 及其修复机制。

在本研究中，我们通过常温等离子体诱变成功地从 AT31 培育出了突变菌株芽孢杆菌 AT31-1。该突变菌株 AT31-1 在 400 mg/L 铅培养基中的去除率高达 48.6%。基因组比较分析表明，突变菌株 AT31-1 有三个突变位点，分别影响外排 RND 转运体渗透酶亚基、反应调节转录因子和一个功能未知的基因。转录分析表明，随着铅浓度从 0 毫克/升升高到 200 毫克/升，再升高到 400 毫克/升，AT31-1 中 283 个基因的表达明显上调，其中包括锌转运 ATPase、亚铁转运蛋白 B、NADH 脱氢酶等。肽代谢过程的基因本体功能和碳代谢的 KEGG 通路被确认与 AT31-1 的极强耐铅能力密切相关。这项研究对细菌的耐铅机制提出了新的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bioresource Technology 工程技术-能源与燃料

CiteScore

20.80

自引率

19.30%

发文量

2013

审稿时长

12 days

期刊介绍： Bioresource Technology publishes original articles, review articles, case studies, and short communications covering the fundamentals, applications, and management of bioresource technology. The journal seeks to advance and disseminate knowledge across various areas related to biomass, biological waste treatment, bioenergy, biotransformations, bioresource systems analysis, and associated conversion or production technologies. Topics include: • Biofuels: liquid and gaseous biofuels production, modeling and economics • Bioprocesses and bioproducts: biocatalysis and fermentations • Biomass and feedstocks utilization: bioconversion of agro-industrial residues • Environmental protection: biological waste treatment • Thermochemical conversion of biomass: combustion, pyrolysis, gasification, catalysis.