Potassium inwardly-rectifying channel subfamily J member 11 (KCNJ11) gene polymorphism in Egyptian type 2 diabetic patients: a single-center study.

IF 2.6 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biology Reports Pub Date : 2024-11-07 DOI:10.1007/s11033-024-10035-4

Abdallah S Abdelazem, Osama Abdelaziz Gaber, Samia Hussein, Fatma Mahmoud Elsaid Nasr, Eman A M Elshorbagy, Sara Mohammed Ibrahim, Abdullah Mohammad Abdel-Hameed, Mai Hamdy Rashad, Amal S El-Shal, Al-Shabrawy M Abdelnabi

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引用次数: 0

Abstract

Background: The KCNJ11 gene belongs to the potassium channel gene family. It has a major role in the secretion of insulin. Genetic variations in KCNJ11 are possibly responsible for the progression of type 2 diabetes mellitus (T2DM). In this study, we investigated the possible correlation between KCNJ11 (rs5210) gene polymorphism and T2DM.

Subjects and method: This study included 92 individuals divided into two groups. Group 1 included 46 type 2 diabetic patients. Group 2 (control group) included 46 healthy participants. A complete history was taken and a full physical examination was performed. Anthropometric data were measured. Laboratory investigations included fasting blood glucose (FBG), two hours post-prandial blood glucose (2HPPBG), glycated hemoglobin (HbA1c), and fasting lipid profile. KCNJ11 (rs5210) single nucleotide polymorphism was detected by polymerase chain reaction restriction-fragment length polymorphism (PCR-RFLP).

Results: Both AG and GG genotypes were associated with increased risk for T2DM (OR 5.2, 95% CI 1.32-20.5, P = 0.01 for AG; and OR 18.2, 95% CI 2.99-31.7, P = 0.002 for GG). Also, the frequency of the G allele was significantly higher in type 2 diabetic patients compared to healthy controls (50% versus 23.9%, respectively). The G allele of rs5210 in KCNJ11 contributed to an increased risk of T2DM (OR 3.18, 95% CI 1.31-7.75, P = 0.01). There was a statistically significant association between increased 2HPPBG and HbA1c levels and the carrier of AG and GG genotypes (P = 0.01 and 0.007, respectively). There was a statistically significant association between total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-c), and high-density lipoprotein-cholesterol (HDL-c) levels and the carrier of AG and GG genotypes (P < 0.001, 0.02, and 0.007, respectively). Regression analysis detected that body mass index (BMI), 2HPPBG, TC, triglycerides (TG), and the G allele of rs5210 in KCNJ11 gene showed a significant association with T2DM (P = 0.004, 0.042, 0.003, 0.006, and 0.01, respectively) while no association was observed with FBG, HbA1c, LDL-c or HDL-c (P = 0.099, 0.123, 0.522, and 0.765, respectively).

Conclusion: KCNJ11 rs5210 genetic polymorphism may raise the risk for the occurrence of T2DM among Egyptians.

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埃及 2 型糖尿病患者内向整流钾通道 J 亚家族成员 11 (KCNJ11) 基因多态性：一项单中心研究。

背景：KCNJ11 基因属于钾通道基因家族：KCNJ11 基因属于钾通道基因家族。它在胰岛素分泌中起着重要作用。KCNJ11 基因的遗传变异可能是导致 2 型糖尿病（T2DM）恶化的原因。本研究探讨了 KCNJ11（rs5210）基因多态性与 T2DM 之间可能存在的相关性：本研究将 92 人分为两组。第一组包括 46 名 2 型糖尿病患者。第二组（对照组）包括 46 名健康参与者。研究人员询问了完整的病史，并进行了全面的身体检查。测量了人体测量数据。实验室检查包括空腹血糖（FBG）、餐后两小时血糖（2HPPBG）、糖化血红蛋白（HbA1c）和空腹血脂。聚合酶链反应限制性片段长度多态性（PCR-RFLP）检测了 KCNJ11（rs5210）单核苷酸多态性：AG和GG基因型均与T2DM风险增加有关（AG的OR为5.2，95% CI为1.32-20.5，P=0.01；GG的OR为18.2，95% CI为2.99-31.7，P=0.002）。此外，与健康对照组相比，2 型糖尿病患者的 G 等位基因频率明显更高（分别为 50%和 23.9%）。KCNJ11 中 rs5210 的 G 等位基因增加了罹患 T2DM 的风险（OR 3.18，95% CI 1.31-7.75，P = 0.01）。2HPPBG 和 HbA1c 水平的升高与 AG 和 GG 基因型携带者之间有统计学意义（P = 0.01 和 0.007）。总胆固醇（TC）、低密度脂蛋白-胆固醇（LDL-c）和高密度脂蛋白-胆固醇（HDL-c）水平与 AG 和 GG 基因型携带者之间存在统计学意义上的显著关联（P 结论：总胆固醇（TC）、低密度脂蛋白-胆固醇（LDL-c）和高密度脂蛋白-胆固醇（HDL-c）水平与 AG 和 GG 基因型携带者之间存在统计学意义上的显著关联：KCNJ11 rs5210 基因多态性可能会增加埃及人发生 T2DM 的风险。

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来源期刊

Molecular Biology Reports 生物-生化与分子生物学

CiteScore

5.00

自引率

0.00%

发文量

1048

审稿时长

5.6 months

期刊介绍： Molecular Biology Reports publishes original research papers and review articles that demonstrate novel molecular and cellular findings in both eukaryotes (animals, plants, algae, funghi) and prokaryotes (bacteria and archaea).The journal publishes results of both fundamental and translational research as well as new techniques that advance experimental progress in the field and presents original research papers, short communications and (mini-) reviews.