Detrimental effects of UV-A radiation on antioxidant capacity and photosynthetic efficiency on a tropical microalga

Abstract

Antioxidants are molecules able to neutralize reactive oxygen species with potential applications in the cosmetic or nutraceutical industries. Abiotic stressors, such as light intensity, ultraviolet (UV) radiation, or nutrient availability, can influence their production. In the perspective of optimizing and understanding the antioxidant capacity of microalgae, we investigated the effects of UV-A radiation on growth, and antioxidant and photosynthetic activities on Tetraselmis, a microalga genus known for its high antioxidant capacity. Cultures were exposed to UV-A radiation alongside to photosynthetically active radiation (PAR) in photobioreactors operated in continuous culture. UV-A exposure affects both the photosynthetic and antioxidant activities of Tetraselmis. Photosynthetic parameters suggest that UV-A has a negative effect on photosynthetic efficiency, particularly on the electron transport chain on short-term exposure (1–2 days). However, a resilience of most physiological parameters was observed over the experiment (10 days) suggesting a photochemical adaption over long-term exposure to UV-A radiation. Concerning the antioxidant capacity, UV-A exposure reduced the antioxidant capacity in Tetraselmis suggesting the use of antioxidant molecules to counteract reactive oxygen species production and prevent damage to photosystem II. Finally, the highest antioxidant capacity never observed with a Tetraselmis sp. was measured in cultures without UV addition, with an IC₅₀ of 2.87 ± 0.24 µg mL⁻¹, a value close to the reference compounds Trolox and α-tocopherol. This study showed the great potential of Tetraselmis as a source of antioxidants under favorable culture condition and without UV-A radiations. Indeed, we discourage the use of UV-A to enhance antioxidant capacity in this species due to its negative impact on it and on the photosynthetic efficiency.