To explore the protective effect and mechanism of KLF7 overexpression on retinal ganglion cells after optic nerve crush in mice.

IF 1.4 4区医学 Q3 OPHTHALMOLOGY

European Journal of Ophthalmology Pub Date : 2024-11-08 DOI:10.1177/11206721241297023

Dihao Hua, Jingsai Gu, Feijia Xie, Yishuang Xu, Zongyuan Li, Hongmei Zheng, Zhen Chen

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引用次数: 0

Abstract

Purpose: To investigate the protective effect and mechanism of Krüppel-like factor 7 (KLF7) on retinal ganglion cells (RGCs) after optic nerve crush (ONC).

Methods: Ninety 10-week-old C57BL/6J mice were randomly assigned to five groups. The blank control group (group A), IVT (intravitreal injection) of KLF7 (group B), IVT of phosphate-buffered saline after ONC (group C), IVT of KLF7 after ONC (group D), and IVT of green fluorescent protein after ONC (group E). Retinal electroretinography and immunofluorescence staining were performed to observe the function and survival rate of RGCs on days 3 and 7 after ONC. The Western Blot determined the expressions of JNK 1, ERK, P38, NF-κB, IL-1, IL-6, and TNF-α seven days after ONC.

Results: After ONC, KLF7 gradually increased from day 3 to day 14, with the peak noted a peak on day 7. On day 7 after ONC, the RGC survival rate in Group D was significantly higher than in groups C (P = 0.028) and E (P = 0.007). The negative PhNR wave amplitude significantly decreased in groups C (P = 0.03) and E (P = 0.04) compared to group D. Moreover, group D also showed significantly higher ERK levels among the groups (all P < 0.01); NF-κB, IL-1, IL-6, and TNF-α expression decreased significantly in group D compared with groups C and E (all P < 0.01).

Conclusions: Overexpression of KLF7 improved the survival rate and function of RGCs in mice ONC models by activating the ERK pathway and inhibiting relevant inflammatory factors. KLF7 may be a promising protective factor against optic nerve damage.

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探索 KLF7 过表达对小鼠视神经挤压后视网膜神经节细胞的保护作用及其机制。

目的：研究视神经挤压（ONC）后Krüppel样因子7（KLF7）对视网膜神经节细胞（RGC）的保护作用及其机制：将90只10周大的C57BL/6J小鼠随机分为5组。空白对照组（A组）、KLF7静脉注射组（B组）、视神经挤压后磷酸盐缓冲液静脉注射组（C组）、视神经挤压后KLF7静脉注射组（D组）和视神经挤压后绿色荧光蛋白静脉注射组（E组）。对ONC后第3天和第7天的RGCs进行视网膜电图和免疫荧光染色，以观察其功能和存活率。Western Blot检测了ONC七天后JNK 1、ERK、P38、NF-κB、IL-1、IL-6和TNF-α的表达：ONC后，KLF7从第3天到第14天逐渐升高，第7天达到峰值。ONC 后第 7 天，D 组的 RGC 存活率明显高于 C 组（P = 0.028）和 E 组（P = 0.007）。与 D 组相比，C 组（P = 0.03）和 E 组（P = 0.04）的 PhNR 负波幅明显下降。此外，在各组中，D 组的 ERK 水平也明显更高（均为 P P 结论）：通过激活ERK通路和抑制相关炎症因子，过表达KLF7可提高小鼠ONC模型中RGCs的存活率和功能。KLF7可能是一种很有前景的视神经损伤保护因子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

European Journal of Ophthalmology 医学-眼科学

CiteScore

3.60

自引率

0.00%

发文量

372

审稿时长

3-8 weeks

期刊介绍： The European Journal of Ophthalmology was founded in 1991 and is issued in print bi-monthly. It publishes only peer-reviewed original research reporting clinical observations and laboratory investigations with clinical relevance focusing on new diagnostic and surgical techniques, instrument and therapy updates, results of clinical trials and research findings.