Characterization of a fish-specific immunoglobulin-like domain-containing protein (Igldcp) in zebrafish (Danio rerio) induced after nodavirus infection

IF 2.7 3区农林科学 Q1 FISHERIES

Developmental and comparative immunology Pub Date : 2024-11-06 DOI:10.1016/j.dci.2024.105285

Nieves Martínez-López, Patricia Pereiro, Amaro Saco, Raquel Lama, Antonio Figueras, Beatriz Novoa

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Abstract

One of the most highly induced genes in zebrafish (Danio rerio) larvae after infection with the nodavirus red-spotted grouper nervous necrosis virus (RGNNV) was a member of the immunoglobulin superfamily (IgSF), which has remained uncharacterized and erroneously annotated in zebrafish and other fish species as galectin 17 (lgals17). We characterized this gene and named it immunoglobulin (Ig)-like domain-containing protein (igldcp), a new member of the IgSF that does not possess orthologs in mammals. Igldcp expression is induced by viral infection and it belongs to the group of interferon-stimulated genes (ISGs). In vitro overexpression of igldcp decreased RGNNV replication, whereas in vivo knockdown of this gene had the opposite effect, resulting in increased larval mortality. RNA-Seq analyses of larvae overexpressing igldcp in the absence or presence of infection with RGNNV showed that the main processes affected by Igldcp could be directly involved in the regulation of various cellular processes associated with the modulation of the immune system.

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斑马鱼（Danio rerio）感染结核病毒后诱导的鱼类特异性免疫球蛋白样结构域含蛋白（Igldcp）的特征。

斑马鱼（Danio rerio）幼体感染结节病毒红斑石斑鱼神经坏死病毒（RGNNV）后，其中一个诱导程度最高的基因是免疫球蛋白超家族（IgSF）的一个成员，该成员在斑马鱼和其他鱼类中一直未被定性和错误注释，如galectin 17（lgals17）。我们确定了该基因的特征，并将其命名为免疫球蛋白（Ig）样结构域含蛋白（igldcp），这是 IgSF 的一个新成员，在哺乳动物中没有同源物。Igldcp 的表达由病毒感染诱导，属于干扰素刺激基因（ISGs）。体外过表达 igldcp 会减少 RGNNV 的复制，而体内敲除该基因则会产生相反的效果，导致幼虫死亡率上升。在没有感染 RGNNV 或感染 RGNNV 的情况下对过表达 igldcp 的幼虫进行的 RNA-Seq 分析表明，受 Igldcp 影响的主要过程可能直接参与调节与免疫系统调节有关的各种细胞过程。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Developmental and comparative immunology 医学-动物学

CiteScore

6.20

自引率

6.90%

发文量

206

审稿时长

49 days

期刊介绍： Developmental and Comparative Immunology (DCI) is an international journal that publishes articles describing original research in all areas of immunology, including comparative aspects of immunity and the evolution and development of the immune system. Manuscripts describing studies of immune systems in both vertebrates and invertebrates are welcome. All levels of immunological investigations are appropriate: organismal, cellular, biochemical and molecular genetics, extending to such fields as aging of the immune system, interaction between the immune and neuroendocrine system and intestinal immunity.