Atomic Force Microscopy and Scanning Ion-Conductance Microscopy for Investigation of Biomechanical Characteristics of Neutrophils.

IF 5.1 2区生物学 Q2 CELL BIOLOGY

Cells Pub Date : 2024-10-23 DOI:10.3390/cells13211757

Mikhail Shvedov, Ekaterina Sherstyukova, Snezhanna Kandrashina, Vladimir Inozemtsev, Viktoria Sergunova

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引用次数: 0

Abstract

Scanning probe microscopy (SPM) is a versatile tool for studying a wide range of materials. It is well suited for investigating living matter, for example, in single-cell neutrophil studies. SPM has been extensively utilized to analyze cell physical properties, providing detailed insights into their structural and functional characteristics at the nanoscale. Its long-standing application in this field highlights its essential role in cell biology and immunology research, significantly contributing to understanding cellular mechanics and interactions. In this review, we discuss the application of SPM techniques, specifically atomic force microscopy (AFM) and scanning ion-conductance microscopy (SICM), to study the fundamental functions of neutrophils. In addition, recent advances in the application of SPM in single-cell immunology are discussed. The application of these techniques allows for obtaining data on the morphology, topography, and mechanical and electrochemical properties of neutrophils with high accuracy.

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原子力显微镜和扫描离子电导显微镜用于研究中性粒细胞的生物力学特征

扫描探针显微镜（SPM）是研究各种材料的多功能工具。它非常适合研究生命物质，例如单细胞中性粒细胞研究。SPM 已被广泛用于分析细胞的物理性质，为了解细胞在纳米尺度上的结构和功能特性提供了详尽的视角。SPM 在这一领域的长期应用凸显了它在细胞生物学和免疫学研究中的重要作用，极大地促进了对细胞力学和相互作用的理解。在这篇综述中，我们将讨论 SPM 技术，特别是原子力显微镜 (AFM) 和扫描离子传导显微镜 (SICM) 在研究中性粒细胞基本功能方面的应用。此外，还讨论了 SPM 在单细胞免疫学中应用的最新进展。应用这些技术可以高精度地获得中性粒细胞的形态、形貌、机械和电化学特性数据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cells Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

9.90

自引率

5.00%

发文量

3472

审稿时长

16 days

期刊介绍： Cells (ISSN 2073-4409) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to cell biology, molecular biology and biophysics. It publishes reviews, research articles, communications and technical notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. Full experimental and/or methodical details must be provided.