Antibiotic resistance genes, colistin-resistant Escherichia coli, and physicochemicals in health care wastewater in Vinh Long General Hospital, Vietnam

IF 2.9 4区环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES

Environmental Monitoring and Assessment Pub Date : 2024-11-11 DOI:10.1007/s10661-024-13345-z

Phong Ngo Thanh, Phong Huynh Xuan, Chinh Dang Van, Ho Phan Long, Huu Huynh Thanh, Hung Tran Do

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Abstract

This study collected ten treated wastewater samples from Vinh Long General Hospital to determine their physicochemical characteristics and antibiotic properties. All treated wastewater samples collected during the monitoring periods complied with national regulations. In addition, these samples did not contain bacteria such as Salmonella, Shigella, and Vibrio cholerae. The investigation yielded a total of 25 Escherichia coli isolates. The E. coli isolates exhibied highest antibiotic resistance rate to ampicillin (100%), followed by ciprofloxacin, amoxicillin/clavulanic acid, and cefazolin (96%, 92%, and 92%, respectively). The resistance rate to fosfomycin was 88%, whereas 80% of the isolates were resistant to sulfamethoxazole-trimethoprim. The resistance rate to gentamicin was 72%, whereas that to imipenem and tetracycline was 52%. In addition, 44% isolates were resistant to chloramphenicol, and 32% of isolates were colistin-resistant. Among analyzed isolates, three were resistant to 10 of 11 tested antibiotics but only displayed intermediate resistance to imipenems (carbapenems). Surprisingly, 23 out of 25 isolates showed a positive ESBL phenotype. Eleven of them had both the bla_TEM and bla_CTX-M-1 group structural genes, while twelve only had the bla_CTX-M-1 group gene. Furthermore, none of the isolated E. coli isolates exhibited the bla_SHV gene. The minimum inhibitory concentration (MIC) of colistin exceeded 4 μg/mL in 8 out of 25 (32%) isolates. Seven of eight isolates (87.5%) carried the mcr-1 gene, while one (12.5%) carried the mcr-8 gene. None of the other mcr (mcr-2 to mcr-9) genes were found.

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越南永隆综合医院医疗废水中的抗生素耐药基因、耐可乐定大肠杆菌和理化物质

本研究从永隆综合医院收集了 10 份经处理的废水样本，以确定其理化特征和抗生素特性。监测期间采集的所有废水处理样本均符合国家规定。此外，这些样本不含沙门氏菌、志贺氏菌和霍乱弧菌等细菌。调查共分离出 25 个大肠埃希氏菌。大肠杆菌对氨苄西林的耐药率最高（100%），其次是环丙沙星、阿莫西林/克拉维酸和头孢唑啉（分别为 96%、92% 和 92%）。对磷霉素的耐药率为 88%，而 80% 的分离株对磺胺甲噁唑-三甲氧苄氨嘧啶有耐药性。对庆大霉素的耐药率为 72%，而对亚胺培南和四环素的耐药率为 52%。此外，44%的分离株对氯霉素耐药，32%的分离株对可乐定耐药。在分析的分离株中，有 3 株对 11 种测试抗生素中的 10 种产生耐药性，但只对亚胺培南（碳青霉烯类）产生中间耐药性。令人惊讶的是，25 个分离株中有 23 个出现了 ESBL 阳性表型。其中 11 个同时具有 blaTEM 和 blaCTX-M-1 组结构基因，12 个只具有 blaCTX-M-1 组基因。此外，分离出的大肠杆菌中没有一个带有 blaSHV 基因。25 个分离菌株中有 8 个（32%）的可乐定最低抑菌浓度（MIC）超过 4 μg/mL。8 个分离株中有 7 个（87.5%）携带 mcr-1 基因，1 个（12.5%）携带 mcr-8 基因。其他 mcr（mcr-2 至 mcr-9）基因均未发现。

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来源期刊

Environmental Monitoring and Assessment 环境科学-环境科学

CiteScore

4.70

自引率

6.70%

发文量

1000

审稿时长

7.3 months

期刊介绍： Environmental Monitoring and Assessment emphasizes technical developments and data arising from environmental monitoring and assessment, the use of scientific principles in the design of monitoring systems at the local, regional and global scales, and the use of monitoring data in assessing the consequences of natural resource management actions and pollution risks to man and the environment.