Investigation and kinetics of hydrogel scaffold with sustained release ciprofloxacin hydrochloride

Abstract

Controlled drug delivery is the mechanism that determines the frequency of the drug as well as the side effect that can be occurred. Many approaches studied the encapsulation of the drug in a polymer matrix to achieve a controlled released drug by the effect crosslinking network. In this work, a loaded hydrogel with ciprofloxacin hydrochloride has been prepared as an incremental effect on the antibacterial properties. So, four formulations named, hydrogel 1, hydrogel 2, hydrogel 3, and hydrogel 4 were prepared using different polymers concentrations (chitosan, gelatin, iota carrageenan (IC), and glutaraldehyde as crosslinking agents) ciprofloxacin hydrochloride was used as a drug model. The obtained data revealed that the hydrogels prepared from chitosan/gelatin/IC hydrogels are porous and have interconnected microstructures. The hydrogels structure was evaluated by ATR-IR spectroscopy, EDX, Swelling rate, SEM, porosity, antibacterial properties as well as drug release and kinetics. The hydrogels fractured at stresses of 0.1665 to 0.0358 Kgf / mm². The swelling rates (SR %) after 24 h. were 412%, 362%, 383%, 375% and 407% for of hydrogel 1, hydrogel 2, hydrogel 3, hydrogel 4 and ciprofloxacin hydrochloride loaded hydrogel 1, respectively. The porosity of the prepared hydrogels was increased as the concentration of IC increased from 72.4 to 78%. Loading of ciprofloxacin hydrochloride was confirmed by the presence of fluorine in the EDX measurement. The ciprofloxacin hydrochloride loaded hydrogel 1 has a sustained release of ciprofloxacin hydrochloride over a time of about 10 h. The first-order kinetic model is the best model for the release of ciprofloxacin hydrochloride from the prepared ciprofloxacin hydrochloride loaded hydrogel 1 with a regression coefficient (R²) of 0.9814. The unloaded samples of hydrogels had almost the same antibacterial properties regardless of the amount of IC. Loading the hydrogel with ciprofloxacin hydrochloride has an incremental effect on the antibacterial properties.