Static headspace-gas chromatography with mass spectrometry for the assessment of the bioaccumulation of volatile organic compounds associated with microplastics in animal tissues

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2024-11-08 DOI:10.1016/j.aca.2024.343412

Rosa Peñalver, María Dolores Pérez-Álvarez, Natalia Campillo, Natalia Arroyo-Manzanares, Pilar Viñas

{"title":"Static headspace-gas chromatography with mass spectrometry for the assessment of the bioaccumulation of volatile organic compounds associated with microplastics in animal tissues","authors":"Rosa Peñalver, María Dolores Pérez-Álvarez, Natalia Campillo, Natalia Arroyo-Manzanares, Pilar Viñas","doi":"10.1016/j.aca.2024.343412","DOIUrl":null,"url":null,"abstract":"<h3>Background</h3>The excessive increase in the production of plastics worldwide and their mismanagement have turned these materials into a threat to the environment and living organisms. Plastics undergo erosion and degradation processes releasing additives, monomers of their formulation, and even generating one of the most worrying emerging pollutants: microplastics. These contaminants have been found in both aquatic and terrestrial ecosystems, so it is clear the need to develop a methodology to determine the accumulation of these pollutants and their associated compounds in living organisms.<h3>Results</h3>This work presents a new combination of static headspace with gas chromatography coupled to mass spectrometry (SHS-GC-MS) for the determination of volatile organic compounds (VOCs) associated with microplastic materials in animal tissue samples. A total of 48 samples were analysed, corresponding to different organs (brain, heart, fat, liver, muscle, lung, kidney) of lamb, pig, rabbit, turkey, chicken, and beef, and 60 VOCs related to the presence of plastics were tentatively identified. Moreover, the identity of 25 VOCs was confirmed and they were also quantified, with contents in the 1.12 to 920 ng g<sup>-1</sup> range. The application of chemometric tools allowed to evaluate the relationship between the type of animal organ and the presence of contaminants associated with microplastics, showing a tendency towards a greater accumulation in kidney and liver. Verification of the origin of the VOCs found was carried out by means of a thermogravimetric analysis - mass spectrometry study.<h3>Significance</h3>The developed analytical approach generates useful and valuable information to better understand the exposure pathways of microplastics and their associated compounds, as well as the related migration and accumulation processes may happen in animal organs. This methodology has demonstrated high sensitivity, precision, robustness, and automation, as well as minimal sample handling.","PeriodicalId":240,"journal":{"name":"Analytica Chimica Acta","volume":null,"pages":null},"PeriodicalIF":5.7000,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytica Chimica Acta","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.aca.2024.343412","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Background

The excessive increase in the production of plastics worldwide and their mismanagement have turned these materials into a threat to the environment and living organisms. Plastics undergo erosion and degradation processes releasing additives, monomers of their formulation, and even generating one of the most worrying emerging pollutants: microplastics. These contaminants have been found in both aquatic and terrestrial ecosystems, so it is clear the need to develop a methodology to determine the accumulation of these pollutants and their associated compounds in living organisms.

Results

This work presents a new combination of static headspace with gas chromatography coupled to mass spectrometry (SHS-GC-MS) for the determination of volatile organic compounds (VOCs) associated with microplastic materials in animal tissue samples. A total of 48 samples were analysed, corresponding to different organs (brain, heart, fat, liver, muscle, lung, kidney) of lamb, pig, rabbit, turkey, chicken, and beef, and 60 VOCs related to the presence of plastics were tentatively identified. Moreover, the identity of 25 VOCs was confirmed and they were also quantified, with contents in the 1.12 to 920 ng g^-1 range. The application of chemometric tools allowed to evaluate the relationship between the type of animal organ and the presence of contaminants associated with microplastics, showing a tendency towards a greater accumulation in kidney and liver. Verification of the origin of the VOCs found was carried out by means of a thermogravimetric analysis - mass spectrometry study.

Significance

The developed analytical approach generates useful and valuable information to better understand the exposure pathways of microplastics and their associated compounds, as well as the related migration and accumulation processes may happen in animal organs. This methodology has demonstrated high sensitivity, precision, robustness, and automation, as well as minimal sample handling.

Abstract Image

查看原文本刊更多论文

利用静态顶空气相色谱-质谱法评估动物组织中与微塑料有关的挥发性有机化合物的生物累积性

背景全球塑料产量的过度增长及其管理不善已使这些材料成为对环境和生物体的威胁。塑料在侵蚀和降解过程中会释放出添加剂、配方单体，甚至产生最令人担忧的新兴污染物之一：微塑料。这些污染物在水生和陆生生态系统中都有发现，因此显然有必要开发一种方法来确定这些污染物及其相关化合物在生物体内的累积情况。结果这项研究提出了一种新的静态顶空与气相色谱-质谱联用（SHS-GC-MS）相结合的方法，用于测定动物组织样本中与微塑料材料相关的挥发性有机化合物（VOCs）。共分析了 48 份样品，分别对应羊肉、猪、兔、火鸡、鸡肉和牛肉的不同器官（脑、心脏、脂肪、肝脏、肌肉、肺、肾脏），初步鉴定出 60 种与塑料存在有关的挥发性有机化合物。此外，还确认了 25 种挥发性有机化合物的身份，并对其进行了定量，含量在 1.12 至 920 纳克 g-1 之间。应用化学计量学工具可以评估动物器官类型与微塑料相关污染物存在之间的关系，结果表明，微塑料在肾脏和肝脏中的累积量更大。通过热重分析-质谱研究，对所发现的挥发性有机化合物的来源进行了验证。重要意义所开发的分析方法提供了有用和有价值的信息，有助于更好地了解微塑料及其相关化合物的暴露途径，以及可能在动物器官中发生的相关迁移和累积过程。该方法具有灵敏度高、精确度高、稳健性高、自动化程度高、样品处理量少等特点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.