Synthesis of MIP@COF@MIL-156@Fe3O4 composite and its application in dispersive solid phase extraction of montelukast in plasma and urine samples

Abstract

In this study, a new magnetic nanocomposite comprised of a molecularly imprinted polymer, metal organic framework, and covalent organic framework was synthesized and used in the dispersive solid-phase extraction of montelukast from plasma and urine samples. The extracted analyte was then analyzed using liquid chromatography-tandem mass spectrometry. The drug extraction process involved adding the synthesized nanocomposite to the biological sample. After vortexing, the solid particles were collected using an external magnet. The adsorbed analyte was subsequently eluted with acetonitrile. By combining of the developed method with liquid chromatography-tandem mass spectrometry, low limits of detection (0.04 and 0.05 ng mL⁻¹ in urine and plasma, respectively) and quantification (0.14 and 0.16 ng mL⁻¹ in urine and plasma, respectively), a wide linear range (0.14-300 and 0.16-300 ng mL⁻¹ in urine and plasma, respectively), acceptable relative standard deviations for intra- and inter-day precisions (≤6.3 %), and good extraction recoveries (75 and 72 % in urine and plasma samples, respectively) were achieved.