Vaginal Mucosal Melanoma Cell Activation in Response to Photon or Carbon Ion Irradiation.

IF 2.1 Q3 ONCOLOGY
International Journal of Particle Therapy Pub Date : 2024-09-29 eCollection Date: 2024-12-01 DOI:10.1016/j.ijpt.2024.100630
Alexandra Charalampopoulou, Amelia Barcellini, Margarita Bistika, Giovanni Battista Ivaldi, Sara Lillo, Giuseppe Magro, Ester Orlandi, Marco Giuseppe Pullia, Sara Ronchi, Paola Tabarelli De Fatis, Angelica Facoetti
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引用次数: 0

Abstract

Purpose: Primary gynecological melanomas are uncommon with lower survival rates compared to cutaneous melanomas. Although melanocytes have been identified in a variety of mucosal membranes, little is known about their interactions or roles inside the mucosa layer. Melanin is a common pigment in nature and is endowed with several peculiar chemical, paramagnetic, and semiconductive characteristics. One of its latest explored functions is its interaction with ionizing radiation as a protective mechanism as well as its implication in the metastatic cascade of tumor cells.

Materials and methods: In this work, we analyzed in vitro the effects of different doses of photon and carbon ion irradiation on dendrite formation, pigmentation, migration, and invasion abilities of human mucosal melanoma cells of the vagina. We evaluated the morphology and melanin production of HMV-II cells exposed to photon and carbon ion beams with single doses between 0.5 and 10 Gy.

Results: Our results showed that irradiation induces dendrite formation or elongation and pigmentation in HMV-II cells in a dose-type-dependent and radiation-type-dependent way but also a decrease in cell motility.

Conclusion: The present study describes for the first time an induction of dendritic formation, melanin production, and alterations in migration and invasion abilities by low-linear energy transfer and high-linear energy transfer radiation in human mucosal melanoma cells, suggesting a radioprotective response to further possible exposures increasing the radioresistance of these cells.

阴道粘膜黑色素瘤细胞对光子或碳离子照射的活化反应
目的:原发性妇科黑色素瘤并不常见,与皮肤黑色素瘤相比,其存活率较低。虽然已在多种粘膜中发现了黑色素细胞,但对它们在粘膜层内的相互作用和作用却知之甚少。黑色素是自然界中常见的色素,具有多种奇特的化学、顺磁性和半导体特性。其最新功能之一是与电离辐射的相互作用,作为一种保护机制,以及在肿瘤细胞转移过程中的作用:在这项工作中,我们在体外分析了不同剂量的光子和碳离子照射对人阴道粘膜黑色素瘤细胞的树突形成、色素沉着、迁移和侵袭能力的影响。我们评估了单次剂量为 0.5 至 10 Gy 的光子和碳离子束照射下 HMV-II 细胞的形态和黑色素生成情况:结果:我们的研究结果表明,辐照诱导 HMV-II 细胞树突形成或伸长以及色素沉着的方式与剂量和辐射类型有关,但同时也会降低细胞的运动能力:本研究首次描述了低线性能量转移和高线性能量转移辐射诱导人粘膜黑色素瘤细胞树突的形成、黑色素的产生以及迁移和侵袭能力的改变,这表明在进一步可能的辐射中,这些细胞的放射保护反应会增加其放射抵抗力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
International Journal of Particle Therapy
International Journal of Particle Therapy Medicine-Radiology, Nuclear Medicine and Imaging
CiteScore
3.70
自引率
5.90%
发文量
23
审稿时长
20 weeks
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