A Sensitive and Quick Fluorescent Sensor for the “Turn-On” Detection and Imaging of Glutathione Based on Sulfur Quantum Dots and MnO2 Nanosheets

IF 3.2 4区化学 Q2 CHEMISTRY, ANALYTICAL

Luminescence Pub Date : 2024-11-07 DOI:10.1002/bio.4929

Zhenlin Hu, Min Cheng, Yuanyan Zhang, Leyao Zhang, Huifeng Xu, Xi Zhu

引用次数: 0

Abstract

Glutathione (GSH) is one of the most abundant bioethanol antioxidants in living cells. Here, a fluorescent probe based on MnO₂ nanosheets and sulfur quantum dots (SQDs) was fabricated. Because of the synergistic effect of IFE and FRET, the fluorescence from SQDs was quenched by MnO₂ nanosheets. In the presence of GSH, the fluorescence of SQDs could be recovered because of the reduction of MnO₂ nanosheets by GSH. The method can detect GSH in the concentration range of 5 ~ 1000 μM with the detection limit as low as 1.26 μM. This quick, easy, and cost-effective sensor could be used for the quantification of GSH in serum samples and the imaging of GSH in Escherichia coli O157:H7.

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基于硫量子点和二氧化锰纳米片的谷胱甘肽 "开启 "检测和成像的灵敏快速荧光传感器。

谷胱甘肽（GSH）是活细胞中最丰富的生物乙醇抗氧化剂之一。在这里，我们制作了一种基于二氧化锰纳米片和硫量子点（SQDs）的荧光探针。由于 IFE 和 FRET 的协同作用，SQDs 的荧光被 MnO2 纳米片淬灭。在有 GSH 存在的情况下，由于 MnO2 纳米片被 GSH 还原，SQDs 的荧光可以恢复。该方法可在 5 ~ 1000 μM 的浓度范围内检测 GSH，检测限低至 1.26 μM。这种快速、简便、经济的传感器可用于血清样品中 GSH 的定量检测和大肠杆菌 O157:H7 中 GSH 的成像检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Luminescence 生物-生化与分子生物学

CiteScore

5.10

自引率

13.80%

发文量

248

审稿时长

3.5 months

期刊介绍： Luminescence provides a forum for the publication of original scientific papers, short communications, technical notes and reviews on fundamental and applied aspects of all forms of luminescence, including bioluminescence, chemiluminescence, electrochemiluminescence, sonoluminescence, triboluminescence, fluorescence, time-resolved fluorescence and phosphorescence. Luminescence publishes papers on assays and analytical methods, instrumentation, mechanistic and synthetic studies, basic biology and chemistry. Luminescence also publishes details of forthcoming meetings, information on new products, and book reviews. A special feature of the Journal is surveys of the recent literature on selected topics in luminescence.