Molecular cloning and characterization of a brassinosteriod biosynthesis-related gene PtoCYP90D1 from Populus tomentosa.

IF 3.5 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Juntao Song, Jie Tan, Tao Long, Yuanshuai Shi, Xu Luo, Yang Liu
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引用次数: 0

Abstract

Brassinosteroids (BRs), one of the major classes of phytohormones are essential for various processes of plant growth, development, and adaptations to biotic and abiotic stresses. In Arabidopsis, AtCYP90D1 acts as a bifunctional cytochrome P450 monooxygenase, catalyzing C-23 hydroxylation in the brassinolide biosynthetic pathway. The present study reports the functional characterizations of PtoCYP90D1, one of the AtCYP90D1 homologous genes from Populus tomentosa. The qRT-PCR analysis showed that PtoCYP90D1 was highly expressed in roots and old leaves. Overexpression of PtoCYP90D1 (PtoCYP90D1-OE) in poplar promoted growth and biomass yield, as well as increased xylem area and cell layers. Transgenic plants exhibited a significant increase in plant height and stem diameter as compared to the wild type. In contrast, the CRISPR/Cas9-generated mutation of PtoCYP90D1 (PtoCYP90D1-KO) resulted in significantly decreased biomass production in transgenic plants. Further studies revealed that cell wall components increased significantly in PtoCYP90D1-OE lines but not in PtoCYP90D1-KO lines, as compared to wild-type plants. Overall, the findings indicate a positive role of PtoCYP90D1 in improving growth rate and elevating biomass production in poplar, which will have positive implications for its versatile industrial or agricultural applications.

番红花杨中铜绿素生物合成相关基因 PtoCYP90D1 的分子克隆和特征描述。
芸苔素甾类化合物(BRs)是植物激素的主要类别之一,对植物生长、发育以及适应生物和非生物胁迫的各种过程至关重要。在拟南芥中,AtCYP90D1 是一种双功能细胞色素 P450 单氧化酶,在黄铜内酯生物合成途径中催化 C-23 羟基化。本研究报告了 PtoCYP90D1 的功能特征,该基因是柿树中 AtCYP90D1 的同源基因之一。qRT-PCR 分析表明,PtoCYP90D1 在根和老叶中高表达。PtoCYP90D1(PtoCYP90D1-OE)在杨树中的过表达促进了生长和生物量产量,并增加了木质部面积和细胞层。与野生型相比,转基因植株的株高和茎径都有显著增加。相反,CRISPR/Cas9 产生的 PtoCYP90D1 突变(PtoCYP90D1-KO)导致转基因植物的生物量产量显著下降。进一步研究发现,与野生型植物相比,PtoCYP90D1-OE 株系的细胞壁成分明显增加,而 PtoCYP90D1-KO 株系的细胞壁成分没有增加。总之,研究结果表明,PtoCYP90D1 在改善杨树生长速度和提高生物量方面发挥了积极作用,这将对杨树在工业或农业方面的广泛应用产生积极影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
BMC Genomics
BMC Genomics 生物-生物工程与应用微生物
CiteScore
7.40
自引率
4.50%
发文量
769
审稿时长
6.4 months
期刊介绍: BMC Genomics is an open access, peer-reviewed journal that considers articles on all aspects of genome-scale analysis, functional genomics, and proteomics. BMC Genomics is part of the BMC series which publishes subject-specific journals focused on the needs of individual research communities across all areas of biology and medicine. We offer an efficient, fair and friendly peer review service, and are committed to publishing all sound science, provided that there is some advance in knowledge presented by the work.
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