Single-enzymatic (AChE/ChOx) colorimetric detection of organophosphorus pesticides based on controllable nanoparticles supported by FeCoCu flower-like structure with peroxidase-like activity

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2024-11-05 DOI:10.1016/j.snb.2024.136878

Ziyu Yan , Jiali Zhong , Ziwen Liao , Peng Xu , Ping Qiu

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引用次数: 0

Abstract

Organophosphorus pesticides (OPs) are widely used but easily persist in the environment, which can impact ecological and biological health. In this paper, the nanoparticles supported by FeCoCu flower-like structure (FeCoCu-FN) was successfully synthesized when the molar ratio of Fe:Co:Cu is 0.6:0.4:0.6, which exhibits strong peroxidase-like activity at high V_m (266.7×10⁻⁸ M/s). Combined with 3,3′,5,5′-tetramethylbenzidine (TMB), two types of colorimetric sensors, acetylcholinesterase (AChE) method and choline oxidase (ChOx) method, were firstly proposed in the same reaction system for detection of OPs based on FeCoCu-FN. The comparisons of both the two assays were conducted in details, such as cost, sensitivity, speed and stability. Moreover, the inhibition effects between parathion methyl (PM) and the two enzymes (AChE and ChOx) were predicted and contrasted using the molecular docking method. It can be found that the free binding energy of AChE with PM is smaller than that of ChOx with PM, which indicates that PM is more easily bound to AChE. On the other hand, the inhibition constant of AChE with PM is smaller than that of ChOx with PM, which illustrates the stronger inhibition effect of PM on AChE. The AChE single-enzyme method has higher sensitivity (LOD=0.011 ng/mL), which was consistent with the result of molecular docking. Two analysis methods were used to detect PM in real samples, and the detection results were validated using t-test and F-test. The results showed that there was no significant difference in accuracy and mean between the two methods, and both methods were reliable.

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基于具有过氧化物酶样活性的 FeCoCu 花状结构支撑的可控纳米粒子的有机磷农药单酶（AChE/ChOx）比色检测方法

有机磷农药（OPs）被广泛使用，但容易在环境中持久存在，影响生态和生物健康。本文成功合成了Fe:Co:Cu摩尔比为0.6:0.4:0.6的FeCoCu花状结构支撑的纳米粒子（FeCoCu-FN），该粒子在高Vm（266.7×10-8 M/s）下表现出很强的过氧化物酶样活性。结合 3,3′,5,5′-四甲基联苯胺（TMB），在同一反应体系中首次提出了基于 FeCoCu-FN 的乙酰胆碱酯酶（AChE）法和胆碱氧化酶（ChOx）法两种比色传感器来检测 OPs。对两种检测方法的成本、灵敏度、速度和稳定性等进行了详细比较。此外，还利用分子对接法预测并对比了甲基对硫磷（PM）与两种酶（AChE 和 ChOx）的抑制作用。结果发现，AChE 与 PM 的自由结合能小于 ChOx 与 PM 的自由结合能，这表明 PM 更容易与 AChE 结合。另一方面，AChE 与 PM 的抑制常数小于 ChOx 与 PM 的抑制常数，说明 PM 对 AChE 的抑制作用更强。AChE 单酶检测法的灵敏度更高（LOD=0.011 ng/mL），这与分子对接的结果一致。采用两种分析方法对实际样品中的 PM 进行了检测，并利用 t 检验和 F 检验对检测结果进行了验证。结果表明，两种方法的准确度和平均值无显著差异，均可靠。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.