Visual aptasensor based on PtNPs/g-C3N4 and DNase I for the field detection of acetamiprid

IF 5.3 2区 化学 Q1 CHEMISTRY, ANALYTICAL
Linhai Wang, Xiao Hu, Guoqing Yao, Qinhua Chen, Sicen Wang
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Abstract

A visual aptasensor based on the combination of graphitic carbon nitride loaded with platinum nanoparticles (PtNPs/g-C3N4) and deoxyribonuclease I (DNase I) was developed for detecting acetamiprid. The prepared PtNPs/g-C3N4 exhibited excellent peroxidase (POD)-like activity, demonstrating the capacity to oxidize the colourless o-phenylenediamine (OPD) to produce the coloured product diaminophenazine (DAP) in the presence of hydrogen peroxide (H2O2). The DNA aptamer of acetamiprid can adsorb onto the surface of PtNPs/g-C3N4, thereby inhibiting its POD activity. The binding of acetamiprid to its aptamer results in the aptamer desorbing from the surface of PtNPs/g-C3N4 and subsequent digestion by DNase I. Ascribed to the synergistic effect of these factors, the aptasensor can achieve the rapid on-site detection of acetamiprid based on the acetamiprid-induced the colour change of the solution just with the smartphone. Furthermore, due to the remarkable fluorescence signal of DAP at 570 nm, the aptasensor under fluorescence mode enables highly sensitive and quantitative detection of acetamiprid with a linear range of 1 ng/mL to 4 µg/mL and a detection limit as low as 0.31 ng/mL. The aptasensor has successfully realized the detection of acetamiprid in river water and cucumber samples, offering a novel perspective for the rapid assessment of environmental pollution and food safety risks associated with acetamiprid residues.

Graphical abstract

基于 PtNPs/g-C3N4 和 DNase I 的视觉感应器,用于现场检测啶虫脒。
本研究开发了一种基于氮化石墨与铂纳米颗粒(PtNPs/g-C3N4)和脱氧核糖核酸酶 I(DNase I)组合的视觉诱导传感器,用于检测啶虫脒。所制备的 PtNPs/g-C3N4 具有出色的过氧化物酶(POD)活性,在过氧化氢(H2O2)的作用下,能氧化无色的邻苯二胺(OPD),生成有色产物二氨基吩嗪(DAP)。啶虫脒的 DNA 合体可吸附到 PtNPs/g-C3N4 表面,从而抑制其 POD 活性。在这些因素的协同作用下,该适配体传感器只需使用智能手机就能根据啶虫脒引起的溶液颜色变化实现对啶虫脒的现场快速检测。此外,由于 DAP 在 570 纳米波长处具有显著的荧光信号,该传感器在荧光模式下可实现对啶虫脒的高灵敏度定量检测,线性范围为 1 ng/mL 至 4 µg/mL,检测限低至 0.31 ng/mL。该传感器成功实现了河水和黄瓜样品中啶虫脒的检测,为快速评估啶虫脒残留带来的环境污染和食品安全风险提供了新的视角。
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来源期刊
Microchimica Acta
Microchimica Acta 化学-分析化学
CiteScore
9.80
自引率
5.30%
发文量
410
审稿时长
2.7 months
期刊介绍: As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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