Development and validation of a controlled heating apparatus for long-term MRI of 3D microfluidic tumor models

IF 3.5 3区工程技术 Q2 ENGINEERING, CHEMICAL

AIChE Journal Pub Date : 2024-11-05 DOI:10.1002/aic.18638

Hassan Alkhadrawi, Kokeb Dese, Dhruvi M. Panchal, Alexander R. Pueschel, Kasey A. Freshwater, Amanda Stewart, Haleigh Henderson, Michael Elkins, Raj T. Dave, Hunter Wilson, John W. Bennewitz, Margaret F. Bennewitz

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引用次数: 0

Abstract

Conventional testing of novel contrast agents for magnetic resonance imaging (MRI) involves cell and animal studies. However, 2D cultures lack dynamic flow and in vivo MRI is limited by regulatory approval of long-term anesthesia use. Microfluidic tumor models (MTMs) offer a cost-effective, reproducible, and high throughput platform for bridging cell and animal models. Yet, MRI of microfluidic devices is challenging, due to small fluid volumes generating low sensitivity. For the first time, an MRI of MTMs was performed at low field strength (1 T) using conventional imaging equipment without microcoils. To enable longitudinal MRI, we developed (1) CHAMP-3 (controlled heating apparatus for microfluidics and portability) which heats MTMs during MRI scans and (2) an MRI-compatible temperature monitoring system. CHAMP-3 maintained chip surface temperature at ~37°C and the media inside at ~35.5°C. Enhanced T₁-weighted MRI contrast was achieved in 3D MTMs with free manganese (Mn²⁺) solutions and Mn²⁺ labeled tumor cells.

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开发并验证用于三维微流控肿瘤模型长期磁共振成像的受控加热装置

用于磁共振成像（MRI）的新型造影剂的传统测试包括细胞和动物研究。然而，二维培养缺乏动态流动性，而且体内磁共振成像受限于长期麻醉的监管审批。微流控肿瘤模型（MTMs）为细胞和动物模型提供了一个具有成本效益、可重复性和高通量的平台。然而，由于流体体积小、灵敏度低，微流控设备的磁共振成像具有挑战性。这是首次使用传统成像设备，在低场强（1 T）下对 MTMs 进行磁共振成像，而不使用微线圈。为实现纵向磁共振成像，我们开发了（1）CHAMP-3（用于微流体和便携性的受控加热设备），它能在磁共振成像扫描期间加热 MTM；（2）与磁共振成像兼容的温度监控系统。CHAMP-3 将芯片表面温度保持在约 37°C，内部介质温度保持在约 35.5°C。用游离锰（Mn2+）溶液和 Mn2+ 标记的肿瘤细胞在三维 MTM 中实现了增强的 T1 加权 MRI 对比度。

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来源期刊

AIChE Journal 工程技术-工程：化工

CiteScore

7.10

自引率

10.80%

发文量

411

审稿时长

3.6 months

期刊介绍： The AIChE Journal is the premier research monthly in chemical engineering and related fields. This peer-reviewed and broad-based journal reports on the most important and latest technological advances in core areas of chemical engineering as well as in other relevant engineering disciplines. To keep abreast with the progressive outlook of the profession, the Journal has been expanding the scope of its editorial contents to include such fast developing areas as biotechnology, electrochemical engineering, and environmental engineering. The AIChE Journal is indeed the global communications vehicle for the world-renowned researchers to exchange top-notch research findings with one another. Subscribing to the AIChE Journal is like having immediate access to nine topical journals in the field. Articles are categorized according to the following topical areas: Biomolecular Engineering, Bioengineering, Biochemicals, Biofuels, and Food Inorganic Materials: Synthesis and Processing Particle Technology and Fluidization Process Systems Engineering Reaction Engineering, Kinetics and Catalysis Separations: Materials, Devices and Processes Soft Materials: Synthesis, Processing and Products Thermodynamics and Molecular-Scale Phenomena Transport Phenomena and Fluid Mechanics.