Resistance toward Triflumezopyrim Related to Overexpression of SfUGT35A1 and SfGSTd2 in Sogatella furcifera (Hemiptera: Delphacidae)

Abstract

Sogatella furcifera is a migratory pest that harms rice cultivation. Triflumezopyrim (TFM), a novel insecticide, effectively controls S. furcifera. To guide its scientific application, we studied the TFM resistance mechanisms in resistant strains. Through cross-resistance determination, we found that the strain had cross-resistance to clothianidin. Synergistic and enzyme activities also demonstrated that the TFM resistance was related to the enhancement of glutathione S-transferases and UDP-glycosyltransferases. Meanwhile, SfGSTd2, SfUGT2, and SfUGT35A1 were invariably upregulated in the TFM-resistant strain. RNAi revealed that SfGSTd2 (mortality increasing 33.3–53.3%) and SfUGT35A1 (increasing 33.3–46.7%) significantly increased sensitivity on TFM, while SfUGT35A1 (increasing 40.0–46.7%) also clearly promoted sensitivity toward clothianidin in S. furcifera. The GAL4/UAS system further verified that the overexpression of SfUGT35A1 is involved in the formation of TFM (mortality descending 2.8–52.8%) and clothianidin resistance (mortality descending 11.1–41.7%). Therefore, all results showed that the overexpression of SfGSTd2- and SfUGT35A1-mediated resistance to TFM in S. furcifera.