Highly Responsive Cryogel Based Sensing Platform by Encapsulating Programmed DNA for Colorimetric Detection of 17β-estradiol

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2024-11-05 DOI:10.1016/j.aca.2024.343394

Yujia Guo, Jiaxuan Xiao, Fengxiang Ai, Xiaofeng Yang, Kun Zeng, Yuhan Wang, Xuesong Li, Zhen Zhang, Hongjun Zhao

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引用次数: 0

Abstract

Responsive hydrogels show obvious superiorities when being employed at detection owing to their polymeric networks and optical properties. Albeit important, the slow response of traditional hydrogels against targets limits their further applications, and enhancing the responsiveness of hydrogel is therefore imperative. Herein, a cryogel with hierarchical structures was designed through adjusting the hydrogel structure by the freezing process to improve the responsive properties, and fabricated for rapid analysis of 17β-estradiol (E2). The cryogel encapsulating the DNA hairpins rapidly adsorbed the complementary DNA isolated from the aptamer in the presence of E2 and activated an efficient hybridization chain reaction (HCR). After integration with a smartphone system, a portable colorimetric sensing platform was created for sensitive detection of E2 with a low detection limit of 0.5 nM. Our work provides a promising way for highly responsive hydrogel-based biosensor design, and broadens their applications in rapid and portable target detection.

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通过封装程序化 DNA 实现高响应性冷凝胶传感平台，用于比色检测 17β-estradiol

响应性水凝胶因其聚合网络和光学特性，在检测中显示出明显的优越性。传统水凝胶虽然重要，但对目标的反应速度较慢，限制了其进一步的应用，因此提高水凝胶的反应速度势在必行。本文通过冷冻过程调整水凝胶结构，设计了一种具有分层结构的冷冻凝胶，以提高其响应特性，并将其制成用于快速分析 17β-estradiol (E2)。在 E2 存在的情况下，包裹 DNA 发夹的冷冻凝胶能迅速吸附从适配体中分离出的互补 DNA，并激活高效的杂交链反应（HCR）。在与智能手机系统集成后，我们创建了一个便携式比色传感平台，用于灵敏检测 E2，检测限低至 0.5 nM。我们的工作为基于水凝胶的高响应生物传感器设计提供了一种前景广阔的途径，并拓宽了其在快速和便携式目标检测方面的应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.