Influence of hormonal factors, number of sexual partners, surgical intervention on gastrointestinal and urogenital microbiota of patients endometriosis

IF 4.7 3区医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL

Archives of Medical Research Pub Date : 2024-11-04 DOI:10.1016/j.arcmed.2024.103112

Han Do , Paula Diaz-Sylvester , Kathleen Groesch , Teresa Wilson , Kristen Delfino , J.Ricardo Loret de Mola , Andrea Braundmeier-Fleming

{"title":"Influence of hormonal factors, number of sexual partners, surgical intervention on gastrointestinal and urogenital microbiota of patients endometriosis","authors":"Han Do , Paula Diaz-Sylvester , Kathleen Groesch , Teresa Wilson , Kristen Delfino , J.Ricardo Loret de Mola , Andrea Braundmeier-Fleming","doi":"10.1016/j.arcmed.2024.103112","DOIUrl":null,"url":null,"abstract":"<div><h3>Problem</h3><div>Endometriosis is associated with gastrointestinal (GI) and urogenital (UG) microbial dysbiosis in patients with endometriosis (P-EOSIS). Sexual partner exposure may contribute to microbial dysbiosis but has not been studied in P-EOSIS. We hypothesized that sexual partner number, hormonal and surgical therapy would affect GI/UG microbial dysbiosis in P-EOSIS.</div></div><div><h3>Methods of Study</h3><div>Urine, fecal and vaginal swabs from control (<em>n</em> = 15) and P-EOSIS (<em>n</em> = 33) were collected on the day of surgery (DOS) and ∼1–3 weeks post-surgical intervention (PSI).</div><div>Control and P-EOSIS were grouped based on hormonal therapy (HT) to determine the effect of HT on microbial profiles, Control (HT <em>n</em> = 8; no HT <em>n</em> = 7) and P-EOSIS (HT <em>n</em> = 18; no HT <em>n</em> = 15). Samples underwent DNA extraction and sequencing of the V4 region of 16S rRNA gene. Sequences were processed using QIIME2 and amplicon sequence variants (ASV) were analyzed for microbial differences. Pearson's and Spearman correlation analyses determined associations among microbial features and sexual partner exposure.</div></div><div><h3>Results</h3><div>P-EOSIS had microbial dysbiosis characterized by unique GI/UG bacteria and altered microbial richness and diversity. Hormonal and surgical intervention in P-EOSIS restored GI microbial diversity. Increased sexual partner exposure decreased GI/UG microbial diversity. P-EOSIS who had 10 or more sexual partners had greater microbial dysbiosis compared to 4–6 partners. Surgical intervention negatively correlated with sexual partner numbers and GI/UG microbial abundance.</div></div><div><h3>Discussion</h3><div>Increased sexual partner exposure may enhance microbial dysbiosis in P-EOSIS and diminish the effectiveness of HT and surgical interventions.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"55 8","pages":"Article 103112"},"PeriodicalIF":4.7000,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archives of Medical Research","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0188440924001632","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MEDICINE, RESEARCH & EXPERIMENTAL","Score":null,"Total":0}

引用次数: 0

Abstract

Problem

Endometriosis is associated with gastrointestinal (GI) and urogenital (UG) microbial dysbiosis in patients with endometriosis (P-EOSIS). Sexual partner exposure may contribute to microbial dysbiosis but has not been studied in P-EOSIS. We hypothesized that sexual partner number, hormonal and surgical therapy would affect GI/UG microbial dysbiosis in P-EOSIS.

Methods of Study

Urine, fecal and vaginal swabs from control (n = 15) and P-EOSIS (n = 33) were collected on the day of surgery (DOS) and ∼1–3 weeks post-surgical intervention (PSI).

Control and P-EOSIS were grouped based on hormonal therapy (HT) to determine the effect of HT on microbial profiles, Control (HT n = 8; no HT n = 7) and P-EOSIS (HT n = 18; no HT n = 15). Samples underwent DNA extraction and sequencing of the V4 region of 16S rRNA gene. Sequences were processed using QIIME2 and amplicon sequence variants (ASV) were analyzed for microbial differences. Pearson's and Spearman correlation analyses determined associations among microbial features and sexual partner exposure.

Results

P-EOSIS had microbial dysbiosis characterized by unique GI/UG bacteria and altered microbial richness and diversity. Hormonal and surgical intervention in P-EOSIS restored GI microbial diversity. Increased sexual partner exposure decreased GI/UG microbial diversity. P-EOSIS who had 10 or more sexual partners had greater microbial dysbiosis compared to 4–6 partners. Surgical intervention negatively correlated with sexual partner numbers and GI/UG microbial abundance.

Discussion

Increased sexual partner exposure may enhance microbial dysbiosis in P-EOSIS and diminish the effectiveness of HT and surgical interventions.

查看原文本刊更多论文

激素因素、性伴侣数量、手术干预对子宫内膜异位症患者胃肠道和泌尿生殖道微生物群的影响

问题子宫内膜异位症患者（P-EOSIS）的胃肠道（GI）和泌尿生殖道（UG）微生物菌群失调与子宫内膜异位症有关。性伴侣接触可能会导致微生物菌群失调，但尚未对 P-EOSIS 进行研究。我们假设性伴侣数量、激素和手术治疗会影响 P-EOSIS 的消化道/子宫内膜微生物菌群失调。研究方法在手术当天（DOS）和手术后 1-3 周（PSI）收集对照组（n = 15）和 P-EOSIS 组（n = 33）的尿液、粪便和阴道拭子。对照组和P-EOSIS组根据激素疗法（HT）分组，以确定HT对微生物特征的影响，对照组（HT n = 8；无HT n = 7）和P-EOSIS组（HT n = 18；无HT n = 15）。样本进行了 DNA 提取和 16S rRNA 基因 V4 区域测序。使用 QIIME2 对序列进行处理，并分析扩增子序列变异（ASV）以确定微生物差异。Pearson和Spearman相关性分析确定了微生物特征与性伴侣暴露之间的关联。对P-EOSIS进行激素和手术干预可恢复消化道微生物多样性。性伴侣接触增加会降低消化道/胃肠道微生物多样性。与 4-6 个性伴侣相比，有 10 个或更多性伴侣的 P-EOSIS 微生物菌群失调程度更高。手术干预与性伴侣数量和 GI/UG 微生物丰富度呈负相关。讨论性伴侣暴露增加可能会加重 P-EOSIS 微生物菌群失调，降低 HT 和手术干预的效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Archives of Medical Research 医学-医学：研究与实验

CiteScore

12.50

自引率

0.00%

发文量

审稿时长

28 days

期刊介绍： Archives of Medical Research serves as a platform for publishing original peer-reviewed medical research, aiming to bridge gaps created by medical specialization. The journal covers three main categories - biomedical, clinical, and epidemiological contributions, along with review articles and preliminary communications. With an international scope, it presents the study of diseases from diverse perspectives, offering the medical community original investigations ranging from molecular biology to clinical epidemiology in a single publication.