Comparative evaluation of the degree of conversion of an 8th-generation bonding agent when applied to normal dentin or caries-affected dentin, pre-treated with MMP inhibitors – An in vitro study

Q1 Medicine

Journal of oral biology and craniofacial research Pub Date : 2024-11-01 DOI:10.1016/j.jobcr.2024.10.010

Neil Vincent Lewis, Shalini Aggarwal (Dr), Prasanna Dhatavkar

{"title":"Comparative evaluation of the degree of conversion of an 8th-generation bonding agent when applied to normal dentin or caries-affected dentin, pre-treated with MMP inhibitors – An in vitro study","authors":"Neil Vincent Lewis, Shalini Aggarwal (Dr), Prasanna Dhatavkar","doi":"10.1016/j.jobcr.2024.10.010","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><div>The degree of conversion of dentin bonding agents contributes to the success of final restorations in adhesive dentistry. It is clinically relevant, as it is directly related to the overall bond strength that maintains these restorations in place and prevents marginal deterioration. However, Matrix metalloproteinases (MMPs) present within the dentin substrate have been known to have a deleterious effect on the bond stability, due to their collagenolytic activity. Inhibition of the effects of MMPs has been studied, and the use of a pre-treatment protocol that includes MMP inhibiting agents before bonding procedures has been explored, and has been found to increase bond strengths. However, the effect that MMP inhibitors have on dental adhesives has not yet been explored, particularly when being used in caries-affected-dentin (CAD). This study aims to report the degree of conversion of an 8th-generation dentin bonding agent in sound dentin (SD) vs caries-affected dentin after pre-treatment using MMP inhibitors.</div></div><div><h3>Materials & methods</h3><div>60 dentin discs were divided into groups of sound and affected dentin, and pretreated with either Cranberry extract or mulberry extract was applied to half of the discs in each group, followed by the application of an eight-generation bonding agent to the pre-treated discs, which were then light cured. The degree of conversion of the adhesive was calculated using ATR-FTIR spectroscopy.</div></div><div><h3>Results</h3><div>The degree of conversion was found to be the highest in the samples of sound dentin which were pre-treated with Mulberry extract.</div></div><div><h3>Conclusion</h3><div>Pre-treatment of the dentin with MMP inhibitors led to an increase in the degree of conversion in sound dentin but not in CAD.</div></div>","PeriodicalId":16609,"journal":{"name":"Journal of oral biology and craniofacial research","volume":"14 6","pages":"Pages 808-814"},"PeriodicalIF":0.0000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of oral biology and craniofacial research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S221242682400157X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

Abstract

Introduction

The degree of conversion of dentin bonding agents contributes to the success of final restorations in adhesive dentistry. It is clinically relevant, as it is directly related to the overall bond strength that maintains these restorations in place and prevents marginal deterioration. However, Matrix metalloproteinases (MMPs) present within the dentin substrate have been known to have a deleterious effect on the bond stability, due to their collagenolytic activity. Inhibition of the effects of MMPs has been studied, and the use of a pre-treatment protocol that includes MMP inhibiting agents before bonding procedures has been explored, and has been found to increase bond strengths. However, the effect that MMP inhibitors have on dental adhesives has not yet been explored, particularly when being used in caries-affected-dentin (CAD). This study aims to report the degree of conversion of an 8th-generation dentin bonding agent in sound dentin (SD) vs caries-affected dentin after pre-treatment using MMP inhibitors.

Materials & methods

60 dentin discs were divided into groups of sound and affected dentin, and pretreated with either Cranberry extract or mulberry extract was applied to half of the discs in each group, followed by the application of an eight-generation bonding agent to the pre-treated discs, which were then light cured. The degree of conversion of the adhesive was calculated using ATR-FTIR spectroscopy.

Results

The degree of conversion was found to be the highest in the samples of sound dentin which were pre-treated with Mulberry extract.

Conclusion

Pre-treatment of the dentin with MMP inhibitors led to an increase in the degree of conversion in sound dentin but not in CAD.

Abstract Image

查看原文本刊更多论文

在使用 MMP 抑制剂预处理的正常牙本质或受龋病影响的牙本质上使用第八代粘接剂时的转化程度比较评估 - 体外研究

导言牙本质粘接剂的转化程度有助于粘接牙科中最终修复体的成功。它与临床相关，因为它直接关系到维持这些修复体就位和防止边缘退化的整体粘接强度。然而，存在于牙本质基质中的基质金属蛋白酶（MMPs）由于具有溶解胶原的活性，对粘接稳定性有有害影响。人们已经对抑制 MMPs 的作用进行了研究，并探索了在粘接程序前使用包括 MMP 抑制剂在内的预处理方案，结果发现这种方法可以提高粘接强度。然而，MMP 抑制剂对牙科粘合剂的影响尚未得到探讨，尤其是在受龋齿影响的牙本质（CAD）中使用时。本研究旨在报告使用 MMP 抑制剂进行预处理后，第八代牙本质粘接剂在健全牙本质（SD）与龋坏影响牙本质中的转化程度。使用 ATR-FTIR 光谱计算粘合剂的转化程度。结果发现，使用桑葚提取物预处理的健全牙本质样本的转化程度最高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of oral biology and craniofacial research Medicine-Otorhinolaryngology

CiteScore

4.90

自引率

0.00%

发文量

133

审稿时长

167 days

期刊介绍： Journal of Oral Biology and Craniofacial Research (JOBCR)is the official journal of the Craniofacial Research Foundation (CRF). The journal aims to provide a common platform for both clinical and translational research and to promote interdisciplinary sciences in craniofacial region. JOBCR publishes content that includes diseases, injuries and defects in the head, neck, face, jaws and the hard and soft tissues of the mouth and jaws and face region; diagnosis and medical management of diseases specific to the orofacial tissues and of oral manifestations of systemic diseases; studies on identifying populations at risk of oral disease or in need of specific care, and comparing regional, environmental, social, and access similarities and differences in dental care between populations; diseases of the mouth and related structures like salivary glands, temporomandibular joints, facial muscles and perioral skin; biomedical engineering, tissue engineering and stem cells. The journal publishes reviews, commentaries, peer-reviewed original research articles, short communication, and case reports.