Proteomic insights of interaction between ichthyotoxic dinoflagellate Karenia mikimotoi and algicidal bacteria Maribacter dokdonensis

IF 5.3 3区环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES

Marine pollution bulletin Pub Date : 2024-11-04 DOI:10.1016/j.marpolbul.2024.117227

Thomas Chun-Hung Lee , Winnie Lam , Nora Fung-Yee Tam , Steven Jing-Liang Xu , Chak-Lam Lee , Fred Wang-Fat Lee

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引用次数: 0

Abstract

Omics technology has been employed in recent research on algicidal bacteria, but previous transcriptomic studies mainly focused on bacteria or algae, neglecting their interaction. This study explores interactions between algicidal bacterium Maribacter dokdonesis P4 and target alga Karenia mikimotoi KMHK using proteomics. Proteomics responses of KMHK after co-culture with P4 in separate compartments of the transwell for 8 and 24 h were evaluated using tandem mass tags (TMT) proteomics, and changes of P4 proteomics were also assessed. Results indicated that essential metabolic processes of KMHK were disrupted after 8 h co-culture with P4. Disturbance of oxidative phosphorylation in mitochondria and electron transport chain in chloroplast raised oxidative stress, leading to endoplasmic reticulum stress and cytoskeleton collapse, and eventual death of KMHK cells. Iron complex outer-membrane receptor protein in P4 was upregulated after co-culture with KMHK for 24 h, suggesting P4 might secrete ferric siderophores, a potential algicidal substance.

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鱼毒甲藻 Karenia mikimotoi 与杀藻细菌 Maribacter dokdonensis 之间相互作用的蛋白质组学启示

近年来有关杀藻细菌的研究已经采用了 Omics 技术，但以往的转录组学研究主要集中在细菌或藻类上，忽略了它们之间的相互作用。本研究利用蛋白质组学探讨了杀藻细菌 P4 与目标藻类 Karenia mikimotoi KMHK 之间的相互作用。使用串联质量标签（TMT）蛋白质组学评估了 KMHK 与 P4 在转孔培养箱的不同隔间共培养 8 小时和 24 小时后的蛋白质组学反应，同时还评估了 P4 蛋白质组学的变化。结果表明，与 P4 共培养 8 小时后，KMHK 的基本代谢过程受到干扰。线粒体氧化磷酸化和叶绿体电子传递链的紊乱增加了氧化应激，导致内质网应激和细胞骨架崩溃，最终导致 KMHK 细胞死亡。与 KMHK 共培养 24 小时后，P4 中的铁复合物外膜受体蛋白上调，表明 P4 可能分泌潜在的杀藻物质--嗜铁苷。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Marine pollution bulletin 环境科学-海洋与淡水生物学

CiteScore

10.20

自引率

15.50%

发文量

1077

审稿时长

68 days

期刊介绍： Marine Pollution Bulletin is concerned with the rational use of maritime and marine resources in estuaries, the seas and oceans, as well as with documenting marine pollution and introducing new forms of measurement and analysis. A wide range of topics are discussed as news, comment, reviews and research reports, not only on effluent disposal and pollution control, but also on the management, economic aspects and protection of the marine environment in general.