Shock wave-pretreated ADMSCs of cell-sheet scaffold (CSS) patched on left ventricular wall (LVW) inhibited LVW remodeling in mini-pig MI ---role CSS on counteracting Laplace's Law of LVW stress: Experimental study.

IF 12.5 2区 医学 Q1 SURGERY
Jiunn-Jye Sheu, Jui-Ning Yeh, Yin-Chia Chen, John Y Chiang, Pei-Hsun Sung, Chi-Ruei Huang, Yi-Chen Li, Hon-Kan Yip
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引用次数: 0

Abstract

Background: We investigated whether shock wave (SW)-pretreated autologous adipocyte-derived mesenchymal stem cells (ADMSCs) seeded in the cell-sheet scaffold (CSS) could inhibit left ventricular (LV) remodeling and improve LV ejection fraction (LVEF) in old myocardial infarction (MI).

Methods: Mini-pigs (n=20) were divided into group 1 (sham-operated control), group 2 (old MI), group 3 (old MI + autologous ADMSCs/1.0×107 in CSS on LV myocardium), and group 4 [old MI + SW (0.12 mJ/mm2 for total 140 shots)-pretreated ADMSCs in CSS on LV myocardium]. Treatments started on day 28 after MI induction. In vivo and in vitro studies were conducted.

Results: Cell viability/relative mitochondria DNA expression/mitochondrial cytochrome C/adenosine triphosphate concentration in ADMCSs and protein expressions of angiogenesis factors (vascular endothelial growth factor [VEGF]/stromal cell-derived factor-1 [SDF-1])/mitochondrial respiratory chain complexes I-IV/oxygen consumption rate were higher in group 4 than in group 3 (P<0.001). By day 180, LVEF and small vessel numbers in the peri-infarct or infarct area were highest in group 1, lowest in group 2, and significantly lower in group 3 than in group 4. In contrast, the LV dimension was opposite to the pattern of change in LVEF in all groups (P<0.0001). The basal/middle/apical infarct and fibrotic areas were inversely related to LVEF in all groups (all P<0.0001). Protein levels of angiogenetic markers (SDF-1α/C-X-C chemokine receptor type 4/VEGF/angiopoietin-1) were significantly and persistently increased from groups 1 to 4. In contrast, protein levels of endothelial-cell markers (von Willebrand factor or endothelial nitric oxide synthase) showed an identical pattern to LVEF in all groups (all P<0.0001).

Conclusion: SW pretreatment of ADMSCs seeded in CSS offered significant benefits in preserving LV performance and ameliorating LV remodeling in mini-pigs with old MI.

经冲击波预处理的细胞片支架(CSS)ADMSCs贴在左心室壁上可抑制小型猪心肌梗死的左心室壁重塑--CSS作用于抵消左心室壁应力的拉普拉斯定律:实验研究。
背景:我们研究了经冲击波(SW)处理的自体脂肪细胞间充质干细胞(ADMSCs)播种于细胞-片支架(CSS)中能否抑制左心室(LV)重塑并改善陈旧性心肌梗死(MI)患者的左心室射血分数(LVEF):将迷你猪(n=20)分为第1组(假手术对照组)、第2组(陈旧性心肌梗死)、第3组(陈旧性心肌梗死+自体ADMSCs/1.0×107,置于左心室心肌的CSS中)和第4组[陈旧性心肌梗死+SW(0.12 mJ/mm2,共140发)-预处理ADMSCs,置于左心室心肌的CSS中]。治疗从诱导 MI 后的第 28 天开始。进行了体内和体外研究:结果:第 4 组 ADMSCs 的细胞存活率/线粒体 DNA 相对表达量/线粒体细胞色素 C/腺苷三磷酸浓度和血管生成因子(血管内皮生长因子 [VEGF] /间质细胞衍生因子-1 [SDF-1])/线粒体呼吸链复合物 I-IV/ 耗氧率的蛋白表达量均高于第 3 组(PC 结论:SW 预处理 ADMSCs 后,ADMSCs 的细胞存活率/线粒体 DNA 相对表达量/线粒体细胞色素 C/腺苷三磷酸浓度均高于第 3 组(PCADMSCs在CSS中播种的SW预处理在保护陈旧性心肌梗死迷你猪的左心室性能和改善左心室重构方面具有显著的益处。
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来源期刊
CiteScore
17.70
自引率
3.30%
发文量
0
审稿时长
6-12 weeks
期刊介绍: The International Journal of Surgery (IJS) has a broad scope, encompassing all surgical specialties. Its primary objective is to facilitate the exchange of crucial ideas and lines of thought between and across these specialties.By doing so, the journal aims to counter the growing trend of increasing sub-specialization, which can result in "tunnel-vision" and the isolation of significant surgical advancements within specific specialties.
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