An inverted tetrahedron-mediated DNA walker for sulfadimethoxine detection

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2024-11-04 DOI:10.1007/s00604-024-06810-6

Tai Ye, Zheng Luo, Yueyue Che, Min Yuan, Hui Cao, Liling Hao, Qian Zhang, Yongxin Xie, Kaisen Zhang, Fei Xu

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Abstract

An inverted DNA tetrahedron–mediated modular DNA walker was developed for the determination of sulfadimethoxine. The inverted DNA tetrahedron scaffold raises several advantages of recognition module including appropriate lateral space, multiple recognition domains, and cost-effectiveness. The proposed inverted DNA tetrahedron-based recognition module exhibited better binding affinity and kinetics toward target antibiotic than that of other DNA tetrahedron counterparts. Upon specific binding with target, the released bipedal DNA walking strand hops to the signal amplification module and moves stochastically with assistant of nicking enzyme. By coupling these two modules, a good linear relationship between the fluorescence intensity of supernatant and the concentration of sulfadimethoxine was achieved in the range 0.1–100 nM, and the limit of detection was 64.7 pM. Furthermore, this modular DNA walker had also successfully applied to spiked honey and milk samples with satisfactory recoveries from 91.5 to 108.8%, demonstrating its practical sensing capability.

Graphical Abstract

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用于磺胺二甲氧嗪检测的倒四面体介导 DNA 步行器。

为测定磺胺二甲氧嗪开发了一种以倒置 DNA 四面体为介导的模块化 DNA 步行器。倒置 DNA 四面体支架提高了识别模块的几个优点，包括适当的横向空间、多个识别域和成本效益。与其他 DNA 四面体相比，基于倒置 DNA 四面体的识别模块对目标抗生素具有更好的结合亲和力和动力学特性。与目标物特异性结合后，释放的双足 DNA 走链跳转到信号放大模块，并在裂解酶的辅助下随机移动。通过耦合这两个模块，上清液的荧光强度与磺胺二甲氧嘧啶的浓度在 0.1-100 nM 的范围内呈良好的线性关系，检测限为 64.7 pM。此外，该模块化 DNA 步行器还成功应用于添加了磺胺二甲氧嘧啶的蜂蜜和牛奶样品，回收率在 91.5%至 108.8%之间，证明了其实用的传感能力。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.