Gilberto Manzo-Sánchez, Luis Carlos Salazar-Licea, Marco Tulio Buenrostro-Nava, Carlos L. Leopardi-Verde, Luciano Martínez-Bolaños, Ignacio Islas-Flores, Mario Orozco-Santos, Blondy Canto-Canché
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引用次数: 0
Abstract
Black Sigatoka disease is a significant threat to banana (Musa spp.) fruit yield and quality. For the control of the fungal pathogen, Pseudocercospora fijiensis, producers currently rely on fungicides such as Quinone outside Inhibitor (QoI). In this study, we examined the resistance status of P. fijiensis to QoI fungicides using 80 isolates from 24 localities in the main banana-producing areas of Mexico (Colima, Michoacán, Tabasco and Chiapas). Resistance was evaluated using the RFLP-PCR mutation assay on Cytochrome b gen (Cytb). The results showed the G143A mutation in three isolates from Chiapas, indicating a relatively low mutation frequency in the sampled areas, where additionally, a microplate bioassay confirmed the resistance to fungicides. We also evaluated the genetic structure and differentiation among the sampled populations, detecting differences between populations within each region and among all populations. Furthermore, our analysis revealed shared haplotypes between resistant populations in Chiapas and nonresistant populations in Michoacán. These findings provide valuable insight into the resistance status of P. fijiensis to QoI fungicides in Mexico and serve as foundation for the development of strategies to manage strobilurin resistance in the country. Overall, this study highlights the importance of monitoring and implementing effective management practices to mitigate the spread of resistant strains.
期刊介绍:
Journal of Phytopathology publishes original and review articles on all scientific aspects of applied phytopathology in agricultural and horticultural crops. Preference is given to contributions improving our understanding of the biotic and abiotic determinants of plant diseases, including epidemics and damage potential, as a basis for innovative disease management, modelling and forecasting. This includes practical aspects and the development of methods for disease diagnosis as well as infection bioassays.
Studies at the population, organism, physiological, biochemical and molecular genetic level are welcome. The journal scope comprises the pathology and epidemiology of plant diseases caused by microbial pathogens, viruses and nematodes.
Accepted papers should advance our conceptual knowledge of plant diseases, rather than presenting descriptive or screening data unrelated to phytopathological mechanisms or functions. Results from unrepeated experimental conditions or data with no or inappropriate statistical processing will not be considered. Authors are encouraged to look at past issues to ensure adherence to the standards of the journal.