Investigation of CST7 and hsa-miR-4793-5p gene expression in breast cancer

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2024-11-01 DOI:10.1016/j.bbrep.2024.101863

Niloufar Sadat Kalaki , Mozhgan Ahmadzadeh , Mandana Dehghan , Venus Shahabi Rabori , Sima Davoudi , Hamed Afkhami

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引用次数: 0

Abstract

Breast cancer (BC) presents as a worldwide challenge, known as the most frequently diagnosed cancer in women. In 2022, BC was diagnosed in 2.3 million women with 670,000 deaths globally. In this research, our objective was to examine the CST7 and has-miR-4793-5p gene expression in BC tumor tissues and adjacent normal tissues.

Using GSE57897 gene expression data from 422 BC samples and 31 breast samples from healthy controls which was based on the Platform GPL18722 (spotted oligonucleotide Homo sapiens microRNA (miRNA) array) in the Gene Expression Omnibus (GEO) and compare with miRNAs with a conserved target location on CST7 mRNA were found using databases. The study population included 60 fresh BC tissue samples and adjacent normal tissues as control. The Quantitative Real-Time PCR was used to evaluate the expression levels of CST7 and has-miR-4793-5p in the breast tissues.

The present study, found that CST7 and hsa-miR-4793-5p were significantly increased in tumoral tissues in compare to normal tissues. Further analysis revealed a remarkable association between CST7 and hsa-miR-4793-5p gene expression alteration. ROC curve analysis demonstrated high accuracy for CST7 expression in BC tumors. Comparison of gene expression between different stages and patient family history showed significant findings. Due to the high sensitivity and specificity of the expression changes of these two genes, they are suitable candidates for further investigations to be considered as part of a diagnosis and prognosis panel.

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乳腺癌中 CST7 和 hsa-miR-4793-5p 基因表达的研究

乳腺癌（BC）是一项世界性难题，是女性最常见的癌症。2022 年，全球有 230 万女性被诊断为乳腺癌，67 万人死亡。本研究旨在检测CST7和has-miR-4793-5p基因在乳腺癌肿瘤组织和邻近正常组织中的表达。我们利用基因表达总库（Gene Expression Omnibus，GEO）中基于 GPL18722 平台（点状寡核苷酸智人 microRNA（miRNA）阵列）的 422 例 BC 样本和 31 例健康对照乳腺样本的 GSE57897 基因表达数据，并利用数据库与 CST7 mRNA 上具有保守靶点位置的 miRNA 进行比较。研究对象包括 60 个新鲜的 BC 组织样本和作为对照的邻近正常组织。本研究发现，与正常组织相比，CST7 和 hsa-miR-4793-5p 在肿瘤组织中的表达明显增加。本研究发现，与正常组织相比，CST7 和 hsa-miR-4793-5p 在肿瘤组织中明显增加，进一步分析表明，CST7 和 hsa-miR-4793-5p 基因表达改变之间存在明显关联。ROC曲线分析表明，CST7在BC肿瘤中的表达具有很高的准确性。不同分期和患者家族史之间的基因表达比较显示了重要的发现。由于这两个基因表达变化的灵敏度和特异性都很高，因此适合作为诊断和预后面板的一部分进行进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.