Effect of cuproptosis on acute kidney injury after cardiopulmonary bypass in diabetic patients.

IF 4.2 3区医学 Q1 ENDOCRINOLOGY & METABOLISM

World Journal of Diabetes Pub Date : 2024-10-15 DOI:10.4239/wjd.v15.i10.2123

Xi-Jin Deng, Yi-Nan Wang, Chuan-Bao Lv, Zhong-Zhi Qiu, Ling-Xin Zhu, Jing-Hui Shi, Si-Ri-Gu-Leng Sana

{"title":"Effect of cuproptosis on acute kidney injury after cardiopulmonary bypass in diabetic patients.","authors":"Xi-Jin Deng, Yi-Nan Wang, Chuan-Bao Lv, Zhong-Zhi Qiu, Ling-Xin Zhu, Jing-Hui Shi, Si-Ri-Gu-Leng Sana","doi":"10.4239/wjd.v15.i10.2123","DOIUrl":null,"url":null,"abstract":"Background: Cardiopulmonary bypass (CPB) is a common procedure in cardiac surgery. CPB is a high-risk factor for acute kidney injury (AKI), and diabetes is also such a factor. Diabetes can lead to copper overload. It is currently unclear whether AKI after CPB in diabetic patients is related to copper overload.Aim: To explore whether the occurrence of CPB-AKI in diabetic patients is associated with cuproptosis.Methods: Blood and urine were collected from clinical diabetic and non-diabetic patients before and after CPB. Levels of copper ion, lactate, glucose, heat shock protein-70 (HSP-70), and dihydrolipoamide dehydrogenase (DLAT) were determined. A diabetic rat model was established and CPB was performed. The rats were assessed for the development of CPB-AKI, and for the association of AKI with cuproptosis by detecting copper levels, iron-sulfur cluster proteins and observation of mitochondrial structure by electron microscopy.Results: CPB resulted in elevations of copper, lactate, HSP-70 and DLAT in blood and urine in both diabetic and non-diabetic patients. CPB was associated with pathologic and mitochondrial damage in the kidneys of diabetic rats. Cuproptosis-related proteins also appeared to be significantly reduced.Conclusion: CPB-AKI is associated with cuproptosis. Diabetes mellitus is an important factor aggravating CPB-AKI and cuproptosis.","PeriodicalId":48607,"journal":{"name":"World Journal of Diabetes","volume":"15 10","pages":"2123-2134"},"PeriodicalIF":4.2000,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11525729/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"World Journal of Diabetes","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4239/wjd.v15.i10.2123","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}

引用次数: 0

Abstract

Background: Cardiopulmonary bypass (CPB) is a common procedure in cardiac surgery. CPB is a high-risk factor for acute kidney injury (AKI), and diabetes is also such a factor. Diabetes can lead to copper overload. It is currently unclear whether AKI after CPB in diabetic patients is related to copper overload.

Aim: To explore whether the occurrence of CPB-AKI in diabetic patients is associated with cuproptosis.

Methods: Blood and urine were collected from clinical diabetic and non-diabetic patients before and after CPB. Levels of copper ion, lactate, glucose, heat shock protein-70 (HSP-70), and dihydrolipoamide dehydrogenase (DLAT) were determined. A diabetic rat model was established and CPB was performed. The rats were assessed for the development of CPB-AKI, and for the association of AKI with cuproptosis by detecting copper levels, iron-sulfur cluster proteins and observation of mitochondrial structure by electron microscopy.

Results: CPB resulted in elevations of copper, lactate, HSP-70 and DLAT in blood and urine in both diabetic and non-diabetic patients. CPB was associated with pathologic and mitochondrial damage in the kidneys of diabetic rats. Cuproptosis-related proteins also appeared to be significantly reduced.

Conclusion: CPB-AKI is associated with cuproptosis. Diabetes mellitus is an important factor aggravating CPB-AKI and cuproptosis.

查看原文本刊更多论文

杯突症对糖尿病患者心肺旁路术后急性肾损伤的影响

背景：心肺旁路术（CPB）是心脏手术中的常见程序。CPB 是急性肾损伤 (AKI) 的高危因素，而糖尿病也是一个高危因素。糖尿病可导致铜超负荷。目的：探讨糖尿病患者发生 CPB-AKI 是否与铜超载有关：方法：收集临床糖尿病和非糖尿病患者在CPB前后的血液和尿液。测定铜离子、乳酸、葡萄糖、热休克蛋白-70（HSP-70）和二氢脂酰胺脱氢酶（DLAT）的水平。建立糖尿病大鼠模型并进行 CPB。通过检测铜含量、铁硫簇蛋白质和电子显微镜观察线粒体结构，评估大鼠是否发生 CPB-AKI，以及 AKI 与杯突变的关系：结果：CPB 导致糖尿病和非糖尿病患者血液和尿液中的铜、乳酸、HSP-70 和 DLAT 升高。CPB 与糖尿病大鼠肾脏的病理和线粒体损伤有关。结论：CPB-AKI 与糖尿病大鼠肾脏的病理和线粒体损伤有关：结论：CPB-AKI 与杯突症有关。糖尿病是加重 CPB-AKI 和杯突症的重要因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

World Journal of Diabetes ENDOCRINOLOGY & METABOLISM-

自引率

2.40%

发文量

909

期刊介绍： The WJD is a high-quality, peer reviewed, open-access journal. The primary task of WJD is to rapidly publish high-quality original articles, reviews, editorials, and case reports in the field of diabetes. In order to promote productive academic communication, the peer review process for the WJD is transparent; to this end, all published manuscripts are accompanied by the anonymized reviewers’ comments as well as the authors’ responses. The primary aims of the WJD are to improve diagnostic, therapeutic and preventive modalities and the skills of clinicians and to guide clinical practice in diabetes. Scope: Diabetes Complications, Experimental Diabetes Mellitus, Type 1 Diabetes Mellitus, Type 2 Diabetes Mellitus, Diabetes, Gestational, Diabetic Angiopathies, Diabetic Cardiomyopathies, Diabetic Coma, Diabetic Ketoacidosis, Diabetic Nephropathies, Diabetic Neuropathies, Donohue Syndrome, Fetal Macrosomia, and Prediabetic State.