Non-natural sialic acid derivatives with o-nitrobenzyl alcohol substituents for light-mediated protein conjugation and cell imaging.

IF 2.9 3区 化学 Q1 CHEMISTRY, ORGANIC
Guo-Biao Zhu, Chen Guo, Xue-Lian Ren, Ming-Zhe Li, Di-Ya Lu, Xi-Le Hu, He Huang, Tony D James, Xiao-Peng He
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引用次数: 0

Abstract

We have synthesized two sialic acid derivatives substituted with an ortho-nitrobenzyl alcohol (o-NBA) group that can undergo light-mediated conjugation with primary amines at their 5- or 9-carbon position. The o-NBA derivatives were shown to react with multiple lysine residues of human serum albumin (HSA) when exposed to 365 nm light irradiation within 10 min. The resulting sugar conjugates were characterized by mass spectroscopy and used for fluorescence-based cell imaging.

具有邻硝基苄醇取代基的非天然硅酸衍生物,用于光介导的蛋白质连接和细胞成像。
我们合成了两种被邻硝基苯甲醇(o-NBA)基团取代的硅酸衍生物,它们可以在 5 碳位或 9 碳位与伯胺发生光介导的共轭反应。在 365 纳米的光照射下,o-NBA 衍生物在 10 分钟内可与人血清白蛋白(HSA)的多个赖氨酸残基发生反应。所产生的糖共轭物通过质谱进行表征,并用于基于荧光的细胞成像。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Organic & Biomolecular Chemistry
Organic & Biomolecular Chemistry 化学-有机化学
CiteScore
5.50
自引率
9.40%
发文量
1056
审稿时长
1.3 months
期刊介绍: The international home of synthetic, physical and biomolecular organic chemistry.
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