Changes in the serum proteome profile of patients with neuroborreliosis, foresters, and patients treated according to ILADS method

IF 3.3 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2024-10-30 DOI:10.1016/j.micpath.2024.107094

Agnieszka Gęgotek , Elżbieta Skrzydlewska , Monika Groth , Piotr Czupryna , Anna Moniuszko-Malinowska

{"title":"Changes in the serum proteome profile of patients with neuroborreliosis, foresters, and patients treated according to ILADS method","authors":"Agnieszka Gęgotek , Elżbieta Skrzydlewska , Monika Groth , Piotr Czupryna , Anna Moniuszko-Malinowska","doi":"10.1016/j.micpath.2024.107094","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><div>The aim of study was to evaluate the changes in proteomic profile of human serum induced by the development of tick-borne neuroborreliosis (NB), before/after therapy, patients treated with prolonged multidrug therapy according to ILADS (International Lyme and Associated Diseases Society), and foresters frequently exposed to tick bites.</div></div><div><h3>Methods</h3><div>A proteomics approach was used to analyze the expression of proteins in serum of patients and sex/age-matched healthy donors. The analysis was performed using SDS-PAGE/LC-MS/MS (Q-Exactive OrbiTrap mass spectrometer).</div></div><div><h3>Results</h3><div>Obtained results indicated changes in the serum proteome of patients with NB putting attention to the proteins involved mainly in calcium transport/metabolism and signaling molecules that differ patients before and after classic therapy. Moreover, ILADS treated patients have different protein distribution than patients from other groups, what is the consequence of prolonged antibiotic therapy. In the case of foresters, the most important result is the increased β-secretase level.</div></div><div><h3>Conclusions</h3><div>Obtained results may contribute to a better understanding of the mechanism of the development of tick-borne diseases, as well as will allow create new opportunities for its rapid and more effective therapy. However, further studies, on larger patients groups, are needed to apply them in clinical practice.</div></div>","PeriodicalId":18599,"journal":{"name":"Microbial pathogenesis","volume":"197 ","pages":"Article 107094"},"PeriodicalIF":3.3000,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbial pathogenesis","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0882401024005618","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"IMMUNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives

The aim of study was to evaluate the changes in proteomic profile of human serum induced by the development of tick-borne neuroborreliosis (NB), before/after therapy, patients treated with prolonged multidrug therapy according to ILADS (International Lyme and Associated Diseases Society), and foresters frequently exposed to tick bites.

Methods

A proteomics approach was used to analyze the expression of proteins in serum of patients and sex/age-matched healthy donors. The analysis was performed using SDS-PAGE/LC-MS/MS (Q-Exactive OrbiTrap mass spectrometer).

Results

Obtained results indicated changes in the serum proteome of patients with NB putting attention to the proteins involved mainly in calcium transport/metabolism and signaling molecules that differ patients before and after classic therapy. Moreover, ILADS treated patients have different protein distribution than patients from other groups, what is the consequence of prolonged antibiotic therapy. In the case of foresters, the most important result is the increased β-secretase level.

Conclusions

Obtained results may contribute to a better understanding of the mechanism of the development of tick-borne diseases, as well as will allow create new opportunities for its rapid and more effective therapy. However, further studies, on larger patients groups, are needed to apply them in clinical practice.

Abstract Image

查看原文本刊更多论文

神经源性疾病患者、森林守护者和按照 ILADS 方法治疗的患者血清蛋白质组的变化。

研究目的本研究旨在评估蜱媒神经性包虫病（NB）发生、治疗前后、根据国际莱姆及相关疾病协会（ILADS）长期接受多种药物治疗的患者以及经常接触蜱虫叮咬的林业人员血清蛋白质组谱的变化：方法：采用蛋白质组学方法分析患者和性别/年龄匹配的健康供体血清中蛋白质的表达。分析采用 SDS-PAGE/LC-MS/MS（Q-Exactive OrbiTrap 质谱仪）进行：结果：研究结果表明，NB 患者血清蛋白质组发生了变化，主要涉及钙转运/代谢和信号分子的蛋白质在经典疗法前后有所不同。此外，ILADS 治疗患者的蛋白质分布与其他组别患者不同，这是长期抗生素治疗的结果。在森林人中，最重要的结果是β-分泌酶水平升高：获得的结果可能有助于更好地了解蜱传疾病的发病机制，并为快速、更有效地治疗蜱传疾病创造新的机会。不过，要将其应用于临床实践，还需要在更大的患者群体中开展进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)