Seahorse assay for the analysis of mitochondrial respiration using Saccharomyces cerevisiae as a model system.

4区 生物学 Q3 Biochemistry, Genetics and Molecular Biology
Methods in enzymology Pub Date : 2024-01-01 Epub Date: 2024-08-24 DOI:10.1016/bs.mie.2024.07.061
Abhishek Kumar, Tejashree Pradip Waingankar, Patrick D'Silva
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引用次数: 0

Abstract

Eukaryotic cells require energy to perform diverse cellular functions critical for survival. Mitochondria are multifunctional organelles that generate energy in the form of Adenosine triphosphate by oxidative phosphorylation, emphasizing their importance to eukaryotic cell viability. The ability of mitochondria to consume oxygen for respiration is a key parameter in assessing mitochondrial health. Therefore, developing new techniques to monitor mitochondrial respiration are crucial for advancing our understanding of organelle functioning. Recently, Seahorse technology has emerged as a valuable tool to analyze various aspects of mitochondrial bioenergetics. Although the Seahorse assay is well established in adherent cell lines and other model organisms, it remains challenging to employ it efficiently in yeast, a powerful genetic system for studying mitochondrial biology. In this chapter, we provide a comprehensive methodology for assessing oxygen consumption rate in baker's yeast using Seahorse.

以酿酒酵母为模型系统分析线粒体呼吸的海马测定法。
真核细胞需要能量来执行对生存至关重要的各种细胞功能。线粒体是一种多功能细胞器,通过氧化磷酸化作用以三磷酸腺苷的形式产生能量,因此线粒体对真核细胞的存活至关重要。线粒体消耗氧气进行呼吸的能力是评估线粒体健康状况的关键参数。因此,开发监测线粒体呼吸的新技术对于增进我们对细胞器功能的了解至关重要。最近,海马技术已成为分析线粒体生物能各个方面的重要工具。尽管海马测定法在粘附细胞系和其他模式生物中已得到广泛应用,但要在酵母这一研究线粒体生物学的强大遗传系统中有效地使用它仍具有挑战性。在本章中,我们将提供一种利用海马法评估面包酵母耗氧率的综合方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Methods in enzymology
Methods in enzymology 生物-生化研究方法
CiteScore
2.90
自引率
0.00%
发文量
308
审稿时长
3-6 weeks
期刊介绍: The critically acclaimed laboratory standard for almost 50 years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Each volume is eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 500 volumes the series contains much material still relevant today and is truly an essential publication for researchers in all fields of life sciences, including microbiology, biochemistry, cancer research and genetics-just to name a few. Five of the 2013 Nobel Laureates have edited or contributed to volumes of MIE.
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