Indirect ELISA for analysis of malignant catarrhal fever virus-specific antibodies in a range of species

IF 2.2 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of virological methods Pub Date : 2024-10-31 DOI:10.1016/j.jviromet.2024.115060

George C. Russell, Ann Percival, Dawn M. Grant

引用次数: 0

Abstract

The culture-attenuated alcelaphine herpesvirus 1 (AlHV-1) C500 strain can be grown to high titre and has been used successfully as a candidate vaccine for wildebeest-associated malignant catarrhal fever (MCF). This vaccine virus was also used to develop an indirect ELISA to allow monitoring of virus-specific antibodies in vaccinated cattle. However the extraction method was expensive and time-consuming, and the resulting test was not suitable for use in sheep. Here we describe an improved antigen extraction method that also broadens the application of the assay, allowing its application to sheep samples. The updated assay was tested using control samples from cattle and sheep, and showed a high level of accuracy in both species. This novel assay should prove to be a useful tool in MCF diagnosis and in evaluation of MCF vaccine responses.

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用于分析各种物种中恶性卡他热病毒特异性抗体的间接酶联免疫吸附试验。

经培养减毒的阿尔卡拉疱疹病毒 1 (AlHV-1) C500 株可生长至高滴度，并已成功用作野蜂相关恶性卡他热 (MCF) 的候选疫苗。这种疫苗病毒还被用于开发一种间接 ELISA 方法，以监测接种疫苗的牛体内的病毒特异性抗体。然而，这种提取方法既昂贵又费时，所得到的检测结果也不适合用于绵羊。在此，我们介绍了一种改进的抗原提取方法，该方法还拓宽了检测方法的应用范围，可用于绵羊样本。我们使用牛和羊的对照样本对更新后的检测方法进行了测试，结果表明该方法在这两个物种中的准确性都很高。这种新型检测方法将被证明是诊断 MCF 和评估 MCF 疫苗反应的有用工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of virological methods 医学-病毒学

CiteScore

5.80

自引率

0.00%

发文量

209

审稿时长

41 days

期刊介绍： The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery. The methods may include, but not limited to, the study of: Viral components and morphology- Virus isolation, propagation and development of viral vectors- Viral pathogenesis, oncogenesis, vaccines and antivirals- Virus replication, host-pathogen interactions and responses- Virus transmission, prevention, control and treatment- Viral metagenomics and virome- Virus ecology, adaption and evolution- Applied virology such as nanotechnology- Viral diagnosis with novelty and comprehensive evaluation. We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.