Polluticaenibacter yanchengensis gen. nov., sp. nov., A Novel Taxon Within the Family Chitinophagaceae Isolated from Activated Sludge.

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2024-11-02 DOI:10.1007/s00284-024-03967-4

Zhuang Ke, Lin Wang, Ying Yang, Yingchun Du, Jian He, Cansheng Yuan

{"title":"Polluticaenibacter yanchengensis gen. nov., sp. nov., A Novel Taxon Within the Family Chitinophagaceae Isolated from Activated Sludge.","authors":"Zhuang Ke, Lin Wang, Ying Yang, Yingchun Du, Jian He, Cansheng Yuan","doi":"10.1007/s00284-024-03967-4","DOIUrl":null,"url":null,"abstract":"A Gram-stain-negative bacterium, designated strain LY-5 T, was isolated from an activated sludge sample collected from a river in Yancheng city, Jiangsu province, China. Cells of strain LY-5 T, were strictly aerobic, non-motile and rod-shaped. Growth occurred at 15-37 °C (optimum, 30 °C), at pH 5.0-9.0 (optimum 7.0) and at 0-3% (w/v) NaCl (optimum, 0%). Phylogenetic analysis based on 16S rRNA gene and genome sequences indicated that strain LY-5 T formed a distinct phyletic branch within the family Chitinophagaceae, with closest relatives being members of the genera Phnomibacter, Aurantisolimonas, and Chitinophaga, sharing 88.5-90.3% sequence similarity. Moreover, the average amino acid identity (AAI) between strain LY-5 T and its closed phylogenetic neighbors was below 70%, indicating it belongs to a novel genus. The predominant cellular fatty acids of LY-5 T were iso-C15:0, iso-C15:1 G and summed feature 3 (C16:1 ω7c and/or C16:1 ω6c), and the only isoprenoid quinone was menaquinone-7 (MK-7). The major polar lipids identified in strain LY-5 T comprised phosphatidylethanolamine, two unidentified aminolipids and five unidentified lipids. The genome size of strain LY-5 T was 4.8 Mbp with a G + C content of 38.9%. Based on the evidence presented in this study, strain LY-5 T represents a novel species of a new genus in the family Chitinophagaceae, for which the name Polluticaenibacter yanchengensis gen. nov., sp. nov. (Type strain LY-5 T = CCTCC AB 2023260 T = KCTC 102218 T) is proposed.","PeriodicalId":11360,"journal":{"name":"Current Microbiology","volume":"81 12","pages":"438"},"PeriodicalIF":2.3000,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00284-024-03967-4","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

A Gram-stain-negative bacterium, designated strain LY-5^T, was isolated from an activated sludge sample collected from a river in Yancheng city, Jiangsu province, China. Cells of strain LY-5^T, were strictly aerobic, non-motile and rod-shaped. Growth occurred at 15-37 °C (optimum, 30 °C), at pH 5.0-9.0 (optimum 7.0) and at 0-3% (w/v) NaCl (optimum, 0%). Phylogenetic analysis based on 16S rRNA gene and genome sequences indicated that strain LY-5^T formed a distinct phyletic branch within the family Chitinophagaceae, with closest relatives being members of the genera Phnomibacter, Aurantisolimonas, and Chitinophaga, sharing 88.5-90.3% sequence similarity. Moreover, the average amino acid identity (AAI) between strain LY-5^T and its closed phylogenetic neighbors was below 70%, indicating it belongs to a novel genus. The predominant cellular fatty acids of LY-5^T were iso-C_15:0, iso-C_15:1 G and summed feature 3 (C_16:1 ω7c and/or C_16:1 ω6c), and the only isoprenoid quinone was menaquinone-7 (MK-7). The major polar lipids identified in strain LY-5^T comprised phosphatidylethanolamine, two unidentified aminolipids and five unidentified lipids. The genome size of strain LY-5^T was 4.8 Mbp with a G + C content of 38.9%. Based on the evidence presented in this study, strain LY-5^T represents a novel species of a new genus in the family Chitinophagaceae, for which the name Polluticaenibacter yanchengensis gen. nov., sp. nov. (Type strain LY-5^T = CCTCC AB 2023260^T = KCTC 102218^T) is proposed.

查看原文本刊更多论文

Polluticaenibacter yanchengensis gen.

从中国江苏省盐城市河道采集的活性污泥样本中分离出一种革兰氏染色阴性细菌，命名为 LY-5 T 菌株。菌株 LY-5 T 的细胞严格需氧，不运动，呈杆状。生长温度为 15-37℃（最适温度为 30℃），pH 值为 5.0-9.0（最适温度为 7.0），NaCl 含量为 0-3%（w/v）（最适温度为 0%）。基于 16S rRNA 基因和基因组序列的系统进化分析表明，菌株 LY-5 T 在甲壳素噬菌科（Chitinophagaceae）中形成了一个独特的植物分支，其近亲是 Phnomibacter 属、Aurantisolimonas 属和 Chitinophaga 属的成员，序列相似度为 88.5%-90.3%。此外，菌株 LY-5 T 与其闭合系统发育近邻的平均氨基酸相同度（AAI）低于 70%，表明它属于一个新属。LY-5 T的主要细胞脂肪酸是异C15:0、异C15:1 G和总特征3（C16:1 ω7c和/或C16:1 ω6c），唯一的异戊烯类醌是脑醌-7（MK-7）。在菌株 LY-5 T 中鉴定出的主要极性脂质包括磷脂酰乙醇胺、两种不明氨基脂质和五种不明脂质。菌株 LY-5 T 的基因组大小为 4.8 Mbp，G+C 含量为 38.9%。根据本研究提供的证据，菌株 LY-5 T 代表了噬甲壳素科中一个新属的新种，其名称为 Polluticaenibacter yanchengensis gen. nov.，sp. nov.（类型菌株 LY-5 T = CCTCC AB 2023260 T = KCTC 102218 T）。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.