Characterization of putative antimicrobial compounds produced by endolichenic Fusarium solani exposed to light treatments.

IF 2.6 2区生物学 Q2 MYCOLOGY

Mycologia Pub Date : 2024-11-01 DOI:10.1080/00275514.2024.2401321

Adeline Su Yien Ting, Peck Ting Gan

{"title":"Characterization of putative antimicrobial compounds produced by endolichenic Fusarium solani exposed to light treatments.","authors":"Adeline Su Yien Ting, Peck Ting Gan","doi":"10.1080/00275514.2024.2401321","DOIUrl":null,"url":null,"abstract":"The endolichenic Fusarium solani (EF5), known to show induced metabolite production when exposed to red and green lights, was selected for characterization of their putative light-regulated bioactive compounds. To achieve this, fractionation was first performed for crude extracts from cultures of F. solani (EF5) incubated in green, red, white-fluorescent light and dark conditions. The extract yielded 12 (dark condition) to 15 (exposed to green, red, and white-fluorescent lights) fractions, and each of the fractions was tested for antimicrobial activities. The fraction (fraction 5) that showed the most promising antimicrobial activity was then subjected to high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) to identify the bioactive compounds. Results revealed detection of two new metabolites from endolichenic F. solani, putatively identified as 8-deoxyjavanicin and fusolanone A, which are known to have antimicrobial properties. This study revealed that red and green lights trigger the production of 8-deoxyjavanicin and fusolanone A, which likely contributed to the antimicrobial properties demonstrated by endolichenic F. solani.","PeriodicalId":18779,"journal":{"name":"Mycologia","volume":" ","pages":"1-10"},"PeriodicalIF":2.6000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycologia","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/00275514.2024.2401321","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MYCOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The endolichenic Fusarium solani (EF5), known to show induced metabolite production when exposed to red and green lights, was selected for characterization of their putative light-regulated bioactive compounds. To achieve this, fractionation was first performed for crude extracts from cultures of F. solani (EF5) incubated in green, red, white-fluorescent light and dark conditions. The extract yielded 12 (dark condition) to 15 (exposed to green, red, and white-fluorescent lights) fractions, and each of the fractions was tested for antimicrobial activities. The fraction (fraction 5) that showed the most promising antimicrobial activity was then subjected to high-performance liquid chromatography (HPLC) and liquid chromatography-mass spectrometry (LC-MS) to identify the bioactive compounds. Results revealed detection of two new metabolites from endolichenic F. solani, putatively identified as 8-deoxyjavanicin and fusolanone A, which are known to have antimicrobial properties. This study revealed that red and green lights trigger the production of 8-deoxyjavanicin and fusolanone A, which likely contributed to the antimicrobial properties demonstrated by endolichenic F. solani.

查看原文本刊更多论文

暴露于光照处理下的内生镰刀菌产生的假定抗菌化合物的特征。

已知内生镰刀菌（EF5）在红光和绿光照射下会产生诱导代谢物，因此我们选择了这种镰刀菌来鉴定其潜在的光调节生物活性化合物。为此，首先对在绿光、红光、白荧光和黑暗条件下培养的 F. solani (EF5) 的粗提取物进行了分馏。提取物产生了 12 个（黑暗条件下）至 15 个（暴露于绿色、红色和白色荧光灯下）馏分，并对每个馏分进行了抗菌活性测试。然后，对抗菌活性最强的馏分（馏分 5）进行高效液相色谱法（HPLC）和液相色谱-质谱法（LC-MS）检测，以确定生物活性化合物。结果显示，从内吸性 F. solani 中检测到了两种新的代谢物，分别为 8-deoxyjavanicin 和 fusolanone A，这两种代谢物具有抗菌特性。这项研究表明，红光和绿光会触发 8-脱氧岩白菜素和扶桑花内酯 A 的产生，这很可能是内吸性茄科真菌具有抗菌特性的原因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Mycologia 生物-真菌学

CiteScore

6.20

自引率

3.60%

发文量

审稿时长

4-8 weeks

期刊介绍： International in coverage, Mycologia presents recent advances in mycology, emphasizing all aspects of the biology of Fungi and fungus-like organisms, including Lichens, Oomycetes and Slime Molds. The Journal emphasizes subjects including applied biology, biochemistry, cell biology, development, ecology, evolution, genetics, genomics, molecular biology, morphology, new techniques, animal or plant pathology, phylogenetics, physiology, aspects of secondary metabolism, systematics, and ultrastructure. In addition to research articles, reviews and short notes, Mycologia also includes invited papers based on presentations from the Annual Conference of the Mycological Society of America, such as Karling Lectures or Presidential Addresses.