Structural characterization and anti-obesity effect of a novel water-soluble galactomannan isolated from Eurotium cristatum

IF 10.7 1区化学 Q1 CHEMISTRY, APPLIED

Carbohydrate Polymers Pub Date : 2024-10-18 DOI:10.1016/j.carbpol.2024.122870

Xiaojie Lu , Yue Jing , Naisheng Zhang , Lei Chen , Jiandong Tai , Yongguo Cao

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Abstract

Obesity is a serious public health challenge worldwide, the present study is aimed to investigate the structural characteristic and anti-obesity effect of a water-soluble galactomannan (PEC) extracted from Eurotium cristatum (E. cristatum). Detailed analysis of the PEC structure showed a weight-average molecular weight of 32,305 Da and a composition of mainly mannose, galactose and small amounts of glucose. Nuclear magnetic resonance spectroscopy combined with methylation analysis indicated that the main chain of PEC is →5)-β-D-Galf-(1 → 6)-α-D-Manp-(1 → glycosidic bond, and the branched chain →2)-α-D-Manp-(1 → through →2,6)-α-D-Manp-(1 → is connected to the main chain by an O-2 bond. Furthermore, PEC was found to ameliorate body weight gain, metabolic disorders, and to modulate the gut microbiota in HFD-fed mice. Fecal microbiota transplantation trial confirmed that PEC prevented obesity development and metabolic disorders by reversing gut dysbiosis in HFD-fed mice. This is the first report of the isolation of PEC from E. cristatum, and the findings suggested that PEC exerted its antiobesity and related beneficial effects by regulating the gut microbiota. In conclusion, as a polysaccharide, PEC could reduce obesity by modulating the gut microbiota and has potential been a prophylactic agent for obesity and related metabolic diseases.

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从 Eurotium cristatum 中分离出的新型水溶性半乳甘露聚糖的结构特征和抗肥胖作用

肥胖症是全球面临的一项严峻的公共卫生挑战，本研究旨在探讨从Eurotium cristatum（E. cristatum）中提取的水溶性半乳甘露聚糖（PEC）的结构特征和抗肥胖作用。对 PEC 结构的详细分析显示，其平均分子量为 32,305 Da，主要成分为甘露糖、半乳糖和少量葡萄糖。核磁共振波谱结合甲基化分析表明，PEC 的主链是→5)-β-D-Galf-(1→6)-α-D-Manp-(1→糖苷键，支链→2)-α-D-Manp-(1→至→2,6)-α-D-Manp-(1→通过 O-2 键与主链相连。此外，研究还发现 PEC 可改善高密度脂蛋白喂养小鼠的体重增加和代谢紊乱，并调节肠道微生物群。粪便微生物群移植试验证实，PEC 可通过逆转高纤维食物喂养小鼠的肠道菌群失调，防止肥胖的发生和代谢紊乱。这是首次从E. cristatum中分离出PEC的报道，研究结果表明PEC通过调节肠道微生物群发挥抗肥胖及相关的有益作用。总之，作为一种多糖，PEC 可通过调节肠道微生物群来减少肥胖，有望成为肥胖及相关代谢疾病的预防药物。

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来源期刊

Carbohydrate Polymers 化学-高分子科学

CiteScore

22.40

自引率

8.00%

发文量

1286

审稿时长

47 days

期刊介绍： Carbohydrate Polymers stands as a prominent journal in the glycoscience field, dedicated to exploring and harnessing the potential of polysaccharides with applications spanning bioenergy, bioplastics, biomaterials, biorefining, chemistry, drug delivery, food, health, nanotechnology, packaging, paper, pharmaceuticals, medicine, oil recovery, textiles, tissue engineering, wood, and various aspects of glycoscience. The journal emphasizes the central role of well-characterized carbohydrate polymers, highlighting their significance as the primary focus rather than a peripheral topic. Each paper must prominently feature at least one named carbohydrate polymer, evident in both citation and title, with a commitment to innovative research that advances scientific knowledge.