Advancing electrochemical sensing: A smart platform for accurate CRP level detection in neonatal septicaemia

Abstract

Neonatal septicaemia is an intricate systemic affliction induced in newborns. A multitude of fungi, viruses, and bacteria elicit this condition. Conventional methodologies such as radiological imaging, microbial testing, blood culture, and inflammatory markers suffer from an array of constraints for prompt detection of infection. This present study illustrates a cutting-edge smart electrochemical biosensing system. The fabricated system was meticulously engineered to discern the concentration of C-reactive protein (CRP). CRP is an irreplaceable prognostic indicator for neonatal sepsis. This work explicates the fabrication of a bioreceptor sensing platform (BioRe Sense) on a screen-printed miniaturized electrode. The surface of the electrode underwent modification with molybdenum disulfide nanosheets (MoS₂NSs). Further, the interface was designed with molecularly imprinted polymer (MIP) targeting CRP. An electrochemical technique carefully examined the electrode at each modification stage. The created sensing interface can detect a wide concentration range from 0.01 nM to 100 nM, with a lower detection limit of 0.01 nM.