Design an Energy-Conserving Pathway for Efficient Biosynthesis of 1,5-Pentanediol and 5-Amino-1-Pentanol.

IF 3.5 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Biotechnology and Bioengineering Pub Date : 2024-10-31 DOI:10.1002/bit.28875

Lin Ma, Chong Xie, Yu Zhang, Wenna Li, Ning An, Xiaolin Shen, Jia Wang, Xinxiao Sun, Qipeng Yuan

引用次数: 0

Abstract

1,5-Pentanediol (1,5-PDO) is an important five-carbon alcohol, widely used in polymer and pharmaceutical industries. Considering the substantial energy (ATP and NADPH) requirements of previous pathways, an energy-conserving artificial pathway with a higher theoretical yield (0.75 mol/mol glucose) was designed and constructed in this study. In this pathway, lysine is converted into 1,5-PDO by decarboxylation, two transamination, and two reduction reactions. For the purpose of full pathway construction, 5-aminopetanal reductase and 5-amino-1-pentanol (5-APO) transaminase were identified and characterized. By implementing strategies such as modular optimization of gene expression, enhancing lysine biosynthesis and increasing NADPH supply, the engineered strains were able to produce 1502.8 mg/L 5-APO and 726.2 mg/L 1,5-PDO in shake flasks and 11.7 g/L 1,5-PDO in a 3 L bioreactor. This work provides a new and promising pathway for the efficient production of 5-APO and 1,5-PDO.

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为 1,5-戊二醇和 5-氨基-1-戊醇的高效生物合成设计节能途径。

1,5-戊二醇（1,5-PDO）是一种重要的五碳醇，广泛应用于聚合物和制药行业。考虑到以前的途径需要大量能量（ATP 和 NADPH），本研究设计并构建了一种理论产量（0.75 摩尔/摩尔葡萄糖）更高的节能人工途径。在这一途径中，赖氨酸通过脱羧、两次转氨基和两次还原反应转化为 1,5-PDO。为了构建完整的途径，对 5-氨基戊醛还原酶和 5-氨基-1-戊醇（5-APO）转氨酶进行了鉴定和表征。通过模块化优化基因表达、加强赖氨酸生物合成和增加 NADPH 供应等策略，工程菌株能够在摇瓶中产生 1502.8 mg/L 5-APO 和 726.2 mg/L 1,5-PDO，在 3 L 生物反应器中产生 11.7 g/L 1,5-PDO。这项工作为高效生产 5-APO 和 1,5-PDO提供了一种新的、前景广阔的途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biotechnology and Bioengineering 工程技术-生物工程与应用微生物

CiteScore

7.90

自引率

5.30%

发文量

280

审稿时长

2.1 months

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