Hypoxia-inducible factor-1α as a biomarker for individuals under hypoxia duration and pattern in fat greenling Hexagrammos otakii

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2024-10-30 DOI:10.1016/j.aqrep.2024.102459

Yu Zhan , Xin Qi , Yiting Wu , Dongxu Gao , Ling Zhao , Shengnan Cao , Zhuang Xue , Wei Wang

{"title":"Hypoxia-inducible factor-1α as a biomarker for individuals under hypoxia duration and pattern in fat greenling Hexagrammos otakii","authors":"Yu Zhan , Xin Qi , Yiting Wu , Dongxu Gao , Ling Zhao , Shengnan Cao , Zhuang Xue , Wei Wang","doi":"10.1016/j.aqrep.2024.102459","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><div>One common environmental stressor in fisheries is hypoxia stress, which frequently causes significant fish mortality and financial losses. This study aimed to examine the potential utility of serum concentrations of hypoxia-inducible factor-1α (HIF-1α) as a biomarker in fat greenlings, <em>Hexagrammos otakii</em>, under hypoxic stress.</div></div><div><h3>Methods</h3><div>The phylogenetic analysis of <em>HIF-1α</em> was carried out. Then, fat greenlings (<em>Hexagrammous otakii</em>) were subjected to hypoxia (DO: 2.2 ± 0.2 mg/L) for 6 h, 12 h, 24 h, 48 h. Upon admission under hypoxia stress, serum HIF-1α concentrations were measured using an enzyme-linked immunosorbent assay (ELISA). The outcomes of the laboratory tests were collected. Then, correlation analysis and regression equation fitting were performed.</div></div><div><h3>Results</h3><div>In this study, <em>HoHIF-1α</em> was determined by bioinformatics analysis. The relative expression of hemoglobinβ2 (<em>HBβ2</em>), lactate dehydrogenase (<em>LDH</em>), endoplasmic reticulum oxidase-1α (<em>ERO-1α</em>), and <em>HIF-1α</em> and the level of HB, LA, ERO-1α and HIF-1α were increased significantly. The hypoxia stress group had considerably greater serum HIF-1α concentrations in comparison to the control group. Furthermore, compared to lactate concentrations, HIF-1α concentrations were linked to a superior predictive capacity for hypoxia stress diagnosis. Then, the correlation analysis result showed that HIF-1α was positively correlated with hemoglobin (HB), ERO-1α, and lactic acid (LA). Meanwhile, HIF-1α has a good linear relationship with HB, ERO-1α, and LA respectively. The HIF-1α concentrations increase of more than 30.27 pg/mL indicates severe hypoxia stress in the <em>H. otakii</em>.</div></div><div><h3>Conclusions</h3><div>HIF-1α was positively correlated with HB, ERO-1α, and LA in <em>H. otakii</em> under hypoxia stress<em>.</em> Serum HIF-1α concentrations are a valuable indicator for hypoxia stress diagnosis and <em>H. otakii</em> recovery status prediction.</div></div>","PeriodicalId":8103,"journal":{"name":"Aquaculture Reports","volume":"39 ","pages":"Article 102459"},"PeriodicalIF":3.2000,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Aquaculture Reports","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2352513424005477","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FISHERIES","Score":null,"Total":0}

引用次数: 0

Abstract

Background

One common environmental stressor in fisheries is hypoxia stress, which frequently causes significant fish mortality and financial losses. This study aimed to examine the potential utility of serum concentrations of hypoxia-inducible factor-1α (HIF-1α) as a biomarker in fat greenlings, Hexagrammos otakii, under hypoxic stress.

Methods

The phylogenetic analysis of HIF-1α was carried out. Then, fat greenlings (Hexagrammous otakii) were subjected to hypoxia (DO: 2.2 ± 0.2 mg/L) for 6 h, 12 h, 24 h, 48 h. Upon admission under hypoxia stress, serum HIF-1α concentrations were measured using an enzyme-linked immunosorbent assay (ELISA). The outcomes of the laboratory tests were collected. Then, correlation analysis and regression equation fitting were performed.

Results

In this study, HoHIF-1α was determined by bioinformatics analysis. The relative expression of hemoglobinβ2 (HBβ2), lactate dehydrogenase (LDH), endoplasmic reticulum oxidase-1α (ERO-1α), and HIF-1α and the level of HB, LA, ERO-1α and HIF-1α were increased significantly. The hypoxia stress group had considerably greater serum HIF-1α concentrations in comparison to the control group. Furthermore, compared to lactate concentrations, HIF-1α concentrations were linked to a superior predictive capacity for hypoxia stress diagnosis. Then, the correlation analysis result showed that HIF-1α was positively correlated with hemoglobin (HB), ERO-1α, and lactic acid (LA). Meanwhile, HIF-1α has a good linear relationship with HB, ERO-1α, and LA respectively. The HIF-1α concentrations increase of more than 30.27 pg/mL indicates severe hypoxia stress in the H. otakii.

Conclusions

HIF-1α was positively correlated with HB, ERO-1α, and LA in H. otakii under hypoxia stress. Serum HIF-1α concentrations are a valuable indicator for hypoxia stress diagnosis and H. otakii recovery status prediction.

查看原文本刊更多论文

缺氧诱导因子-1α作为胖青蛙缺氧持续时间和模式的生物标志物

背景渔业中常见的一种环境胁迫是缺氧胁迫，它经常导致鱼类大量死亡和经济损失。本研究旨在探讨缺氧诱导因子-1α（HIF-1α）血清浓度作为缺氧胁迫下肥青鱼生物标志物的潜在作用。将肥育蛙分别置于缺氧环境（溶解氧：2.2 ± 0.2 mg/L）6 h、12 h、24 h、48 h。在低氧胁迫下入场时，使用酶联免疫吸附试验（ELISA）测量血清中 HIF-1α 的浓度。收集实验室检测结果。结果在这项研究中，HoHIF-1α 是通过生物信息学分析确定的。血红蛋白β2（HBβ2）、乳酸脱氢酶（LDH）、内质网氧化酶-1α（ERO-1α）和 HIF-1α 的相对表达量以及 HB、LA、ERO-1α 和 HIF-1α 的水平均显著升高。与对照组相比，缺氧应激组的血清 HIF-1α 浓度要高得多。此外，与乳酸浓度相比，HIF-1α浓度对缺氧应激诊断的预测能力更强。相关分析结果显示，HIF-1α与血红蛋白（HB）、ERO-1α和乳酸（LA）呈正相关。同时，HIF-1α分别与HB、ERO-1α和LA呈良好的线性关系。结论在缺氧应激状态下，HIF-1α与HB、ERO-1α和LA呈正相关。血清HIF-1α浓度是诊断缺氧应激和预测奥塔克伊蚊恢复状况的重要指标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.