Stable, multigenerational transmission of the bean seed microbiome despite abiotic stress.

IF 5 2区 生物学 Q1 MICROBIOLOGY
mSystems Pub Date : 2024-10-30 DOI:10.1128/msystems.00951-24
Abby Sulesky-Grieb, Marie Simonin, A Fina Bintarti, Brice Marolleau, Matthieu Barret, Ashley Shade
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引用次数: 0

Abstract

Microbiota that originate in the seed can have consequences for the education of the plant immune system, competitive exclusion of pathogens from the host tissue, and host access to critical nutrients. Our research objective was to investigate the consequences of the environmental conditions of the parent plant for bacterial seed microbiome assembly and transmission across plant generations. Using a fully factorial, three-generational experimental design, we investigated endophytic seed bacterial communities of common bean lines (Phaseolus vulgaris L.) grown in the growth chamber and exposed to either control conditions, drought, or excess nutrients at each generation. We applied 16S rRNA microbiome profiling to the seed endophytes and measured plant health outcomes. We discovered stable transmission of 22 bacterial members, regardless of the parental plant condition. This study shows the maintenance of bacterial members of the plant microbiome across generations, even under environmental stress. Overall, this work provides insights into the ability of plants to safeguard microbiome members, which has implications for crop microbiome management in the face of climate change.IMPORTANCESeed microbiomes initiate plant microbiome assembly and thus have critical implications for the healthy development and performance of crops. However, the consequences of environmental conditions of the parent plant for seed microbiome assembly and transmission are unknown, but this is critical information, given the intensifying stressors that crops face as the climate crisis accelerates. This study provides insights into the maintenance of plant microbiomes across generations, with implications for durable plant microbiome maintenance in agriculture on the changing planet.

尽管存在非生物胁迫,豆类种子微生物群仍能稳定、多代传递。
起源于种子的微生物群会影响植物免疫系统的教育、宿主组织对病原体的竞争性排斥以及宿主对关键营养物质的获取。我们的研究目标是调查母本植物的环境条件对细菌种子微生物组的组装和植物跨代传播的影响。我们采用全因子三代实验设计,研究了在生长室中生长的普通豆类品系(Phaseolus vulgaris L.)的内生种子细菌群落,每一代都暴露在对照条件、干旱或过量养分下。我们对种子内生菌进行了 16S rRNA 微生物组分析,并测量了植物健康结果。我们发现,无论亲本植物的状况如何,都有 22 种细菌成员在稳定传播。这项研究表明,即使在环境压力下,植物微生物组中的细菌成员也能跨代维持。重要意义种子微生物组启动了植物微生物组的组装,因此对作物的健康发育和表现有着至关重要的影响。然而,母本植物的环境条件对种子微生物组的组装和传播所产生的影响尚不清楚,但鉴于随着气候危机的加速,农作物所面临的压力日益加剧,这是至关重要的信息。这项研究为植物微生物组的跨代维持提供了见解,对在不断变化的地球上持久维持农业中的植物微生物组具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
mSystems
mSystems Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
10.50
自引率
3.10%
发文量
308
审稿时长
13 weeks
期刊介绍: mSystems™ will publish preeminent work that stems from applying technologies for high-throughput analyses to achieve insights into the metabolic and regulatory systems at the scale of both the single cell and microbial communities. The scope of mSystems™ encompasses all important biological and biochemical findings drawn from analyses of large data sets, as well as new computational approaches for deriving these insights. mSystems™ will welcome submissions from researchers who focus on the microbiome, genomics, metagenomics, transcriptomics, metabolomics, proteomics, glycomics, bioinformatics, and computational microbiology. mSystems™ will provide streamlined decisions, while carrying on ASM''s tradition of rigorous peer review.
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