Soluble Guanylate Cyclase Dysfunction and Nitric Oxide Pathway in Chronic Rhinosinusitis With Nasal Polyps: Predictive Markers for Postoperative Recurrence.
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引用次数: 0
Abstract
Background: Elevated nitric oxide (NO) levels have been linked to a heightened risk of recurrence in patients with chronic rhinosinusitis with nasal polyps (CRSwNP). However, the precise influence of NO on CRSwNP recurrence remains unclear.
Objective: This study seeks to elucidate the relationship between NO levels and the risk of CRSwNP recurrence.
Methods: A protein chip array analysis was conducted to identify differentially expressed inflammatory mediators in the nasal tissues between patients with CRSwNP and healthy controls (HC). Differentially expressed proteins were analyzed, and bioinformatics analysis was used to predict the potential functions and pathways of these proteins. Western blotting (WB) and immunohistochemistry were employed to validate the candidate proteins in 2 independent cohorts. Receiver-operating characteristic (ROC) curves were employed to assess the abilities of target proteins for predicting the postoperative recurrence of CRSwNP.
Results: Twelve differentially expressed proteins were identified between the CRSwNP and HC groups. Notably, differentially expressed proteins exhibited high expression of the biological process term "positive regulation of nitric oxide-mediated signal transduction" (P < .05). WB and immunohistochemistry results demonstrated that guanylate cyclase 1 soluble subunit alpha 1 (GUCY1A1), GUCY1A2, nitric oxide synthase 1 adaptor protein, epidermal growth factor receptor, and insulin were found to be upregulated in the CRSwNP group compared to the HC group (P < .05). Moreover, elevated levels of GUCY1A2 and GUCY1A1 were observed to be associated with an increased risk of CRSwNP recurrence (P < .05), and ROC curve analysis confirmed their effectiveness as predictors for postoperative recurrence (P < .05).
Conclusion: Our findings revealed that CRSwNP exhibited a distinct tissue protein profile, with soluble guanylate cyclase dysfunction and the nitric oxide pathway implicated in the underlying pathological mechanisms. The discovery-validation results suggested that GUCY1A1 and GUCY1A2 were promising predictors for postoperative recurrence in patients with CRSwNP.
背景：一氧化氮（NO）水平升高与慢性鼻炎伴鼻息肉（CRSwNP）患者复发风险升高有关。然而，一氧化氮对 CRSwNP 复发的确切影响仍不清楚：本研究旨在阐明 NO 水平与 CRSwNP 复发风险之间的关系：方法：通过蛋白芯片阵列分析，确定 CRSwNP 患者与健康对照组（HC）鼻腔组织中不同表达的炎症介质。对差异表达的蛋白质进行分析，并利用生物信息学分析预测这些蛋白质的潜在功能和通路。在两个独立队列中采用了 Western 印迹（WB）和免疫组织化学方法来验证候选蛋白。采用接收方操作特征曲线（ROC）评估目标蛋白预测CRSwNP术后复发的能力：结果：在 CRSwNP 组和 HC 组之间发现了 12 个差异表达蛋白。值得注意的是，差异表达的蛋白质在生物学过程术语 "一氧化氮介导的信号转导的正向调节"（P P P P P 结论）中表现出高表达：我们的研究结果表明，CRSwNP 表现出独特的组织蛋白谱，可溶性鸟苷酸环化酶功能障碍和一氧化氮通路与潜在的病理机制有关。发现-验证结果表明，GUCY1A1 和 GUCY1A2 是 CRSwNP 患者术后复发的预测因子。
期刊介绍：
The American Journal of Rhinology & Allergy is a peer-reviewed, scientific publication committed to expanding knowledge and publishing the best clinical and basic research within the fields of Rhinology & Allergy. Its focus is to publish information which contributes to improved quality of care for patients with nasal and sinus disorders. Its primary readership consists of otolaryngologists, allergists, and plastic surgeons. Published material includes peer-reviewed original research, clinical trials, and review articles.
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