Modulation of Terpenoid Indole Alkaloid Biosynthesis in Catharanthus roseus by Sphingomonas Sp Y503 via the CrMAPKKK1-CrMAPKK1/CrMAPKK2-CrMPK3 Signaling Cascade.

IF 6 1区生物学 Q1 PLANT SCIENCES

Plant, Cell & Environment Pub Date : 2024-10-30 DOI:10.1111/pce.15253

Xiaoxiao Gao, Xiaona Zhu, Zhiqin Wang, Xuejing Liu, Rui Guo, Jing Luan, Zhiwen Liu, Fang Yu

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引用次数: 0

Abstract

Catharanthus roseus is a highly relevant model for investigating plant defense mechanisms and the biosynthesis of therapeutically valuable compounds, including terpenoid indole alkaloids (TIAs). It has been demonstrated that beneficial microbial interactions can regulate TIA biosynthesis in C. roseus, highlighting the need to fully comprehend the molecular mechanisms involved to efficiently implement eco-friendly strategies. This study explores the effects of a novel microbial strain, Y503, identified as Sphingomonas sp., on TIA production and the underlying mechanisms in C. roseus. Through bioinformatics analysis, we have identified 17 MAPKKKs, 7 MAPKKs, and 13 MAPKs within the C. roseus genome. Further investigation has verified the presence of the MAPK module (CrMAPKKK1-CrMAPKK1/CrMAPKK2-CrMPK3) mediating Y503 in regulating TIA biosynthesis in C. roseus. This study provides foundational information for strengthening the plant defense system in C. roseus through advantageous microbial interactions, which could contribute to the sustainable cultivation of medicinal plants such as C. roseus.

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鞘氨醇单胞菌 Sp Y503 通过 CrMAPKK1-CrMAPKK1/CrMAPKK2-CrMPK3 信号级联调控石竹属植物的萜类吲哚生物合成。

蔷薇是研究植物防御机制和包括萜类吲哚生物碱（TIAs）在内的有治疗价值化合物的生物合成的高度相关模型。研究表明，有益微生物的相互作用可以调节蔷薇中吲哚生物碱的生物合成，这凸显了充分理解相关分子机制以有效实施生态友好战略的必要性。本研究探讨了一种新型微生物菌株 Y503（被鉴定为鞘氨醇单胞菌 sp.）对 C. roseus 中 TIA 生产的影响及其内在机制。通过生物信息学分析，我们在玫瑰茄基因组中发现了 17 个 MAPKKs、7 个 MAPKKs 和 13 个 MAPKs。进一步的调查验证了 MAPK 模块（CrMAPKK1-CrMAPKK1/CrMAPKK2-CrMPK3）介导 Y503 在 C. roseus 中调节 TIA 生物合成的存在。这项研究为通过有利的微生物相互作用加强蔷薇科植物的植物防御系统提供了基础信息，有助于蔷薇科植物等药用植物的可持续栽培。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant, Cell & Environment 生物-植物科学

CiteScore

13.30

自引率

4.10%

发文量

253

审稿时长

1.8 months

期刊介绍： Plant, Cell & Environment is a premier plant science journal, offering valuable insights into plant responses to their environment. Committed to publishing high-quality theoretical and experimental research, the journal covers a broad spectrum of factors, spanning from molecular to community levels. Researchers exploring various aspects of plant biology, physiology, and ecology contribute to the journal's comprehensive understanding of plant-environment interactions.