Integrated membranes system for water application in microbiology/molecular biology

IF 5.7 3区环境科学与生态学 Q1 WATER RESOURCES

Applied Water Science Pub Date : 2024-10-30 DOI:10.1007/s13201-024-02294-0

Heba Abdallah, Dina Nadeem Abd-Elshafy, Marwa Shalaby, Kareem Awad, Rola Nadeem, Ahmed Shaban, Mahmoud Mohamed Bahgat

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Abstract

An integrated membrane filtration system was developed to make water purity suitable for microbiology/molecular biology research. Water samples were collected from outlets in different buildings of the National Research Center and analyzed for their composition before filtration. An integrated membrane system was developed based on mathematical modeling. Flat sheet membranes were produced, including microfiltration, ultrafiltration, and nanofiltration membranes. The flat sheet membranes were converted to a spiral wound module filter to simulate the local market filters and applied in the integrated membrane system that was designed and installed. The produced water was analyzed and compared to molecular-grade water used in different molecular biology/microbiology applications. Both PCR, agarose gel electrophoresis, bacterial liquid cultures, and viral propagation indicated that treated water using the herein-developed system exhibited comparable performance to the molecular grade water provided with imported reagent kits. So, this research can offer a promising solution for producing high-quality water suitable for sensitive laboratory applications.

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用于微生物学/分子生物学水应用的集成膜系统

为了使水的纯度适合微生物学/分子生物学研究，开发了一种集成膜过滤系统。从国家研究中心不同建筑的出水口收集水样，并在过滤前对其成分进行分析。在数学建模的基础上开发了一个集成膜系统。生产出了平板膜，包括微滤、超滤和纳滤膜。平板膜被转换成螺旋缠绕组件过滤器，以模拟当地市场上的过滤器，并应用于设计和安装的集成膜系统中。对生产的水进行了分析，并与不同分子生物学/微生物学应用中使用的分子级水进行了比较。聚合酶链式反应、琼脂糖凝胶电泳、细菌液体培养和病毒繁殖均表明，使用本研究开发的系统处理过的水与进口试剂盒提供的分子级水性能相当。因此，这项研究为生产适用于敏感实验室应用的优质水提供了一种前景广阔的解决方案。

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