Fluorescence spatial anisotropy of emission dipoles in an orthogonal imaging system

IF 3.5 2区工程技术 Q2 OPTICS

Optics and Lasers in Engineering Pub Date : 2024-10-22 DOI:10.1016/j.optlaseng.2024.108649

Yahui Song , Xin Xu , Jixiang Wang , Lina Xing , Xin Zhang , Guohua Shi , Hong Ye

引用次数: 0

Abstract

In this study, we explore the relatively unexplored spatially anisotropic fluorescence emission induced by rotationally polarized excitation light in orthogonal imaging systems, a phenomenon that is particularly pronounced in orthogonal setups compared to coaxial configurations. Despite its significance, this aspect has been largely neglected, given the prevalent use of coaxial Fluorescence Polarization Microscope (FPM) setups. Our research endeavors to bridge this gap by formulating a physical model to investigate the polarization-dependent emission of dipoles and the corresponding fluorescence signal within an orthogonal imaging configuration. Additionally, we introduce the Fluorescence Spatial Anisotropy Index (FSAI) to quantify fluorescence spatial anisotropy.

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正交成像系统中发射偶极子的荧光空间各向异性

在本研究中，我们探讨了正交成像系统中旋转偏振激发光诱导的空间各向异性荧光发射，这种现象在正交设置中与同轴配置相比尤为明显。尽管这一现象非常重要，但由于同轴荧光偏振显微镜（FPM）装置的普遍使用，它在很大程度上被忽视了。我们的研究通过建立一个物理模型来研究偶极子的偏振相关发射以及正交成像配置中相应的荧光信号，努力弥补这一差距。此外，我们还引入了荧光空间各向异性指数（FSAI）来量化荧光空间各向异性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Optics and Lasers in Engineering 工程技术-光学

CiteScore

8.90

自引率

8.70%

发文量

384

审稿时长

42 days

期刊介绍： Optics and Lasers in Engineering aims at providing an international forum for the interchange of information on the development of optical techniques and laser technology in engineering. Emphasis is placed on contributions targeted at the practical use of methods and devices, the development and enhancement of solutions and new theoretical concepts for experimental methods. Optics and Lasers in Engineering reflects the main areas in which optical methods are being used and developed for an engineering environment. Manuscripts should offer clear evidence of novelty and significance. Papers focusing on parameter optimization or computational issues are not suitable. Similarly, papers focussed on an application rather than the optical method fall outside the journal''s scope. The scope of the journal is defined to include the following: -Optical Metrology- Optical Methods for 3D visualization and virtual engineering- Optical Techniques for Microsystems- Imaging, Microscopy and Adaptive Optics- Computational Imaging- Laser methods in manufacturing- Integrated optical and photonic sensors- Optics and Photonics in Life Science- Hyperspectral and spectroscopic methods- Infrared and Terahertz techniques