Hamza Elbaza , Hanaa Mabroum , El Mehdi Toufik , Badre Eddine Halimi , Yousra Hamdan , Rachid El Fatimy , Hicham Ben youcef , Christèle Combes , Allal Barroug , Hassan Noukrati
{"title":"Delivery of sodium fusidate from alginate-reinforced, carbonated apatite cement: Physicochemical properties, release behavior, antibacterial and cytotoxicity properties","authors":"Hamza Elbaza , Hanaa Mabroum , El Mehdi Toufik , Badre Eddine Halimi , Yousra Hamdan , Rachid El Fatimy , Hicham Ben youcef , Christèle Combes , Allal Barroug , Hassan Noukrati","doi":"10.1016/j.mtla.2024.102248","DOIUrl":null,"url":null,"abstract":"<div><div>The present work focuses on the development of composite cements based on dicalcium phosphate dihydrate (DCPD), calcium carbonate CaCO<sub>3</sub>, sodium alginate (AG), and sodium fusidate (FS). The effect of AG, setting accelerator (0.5 M of Na<sub>2</sub>HPO<sub>4</sub>), and antibacterial agent (FS) on the features (setting ability, injectability, cohesion, and compressive strength) of DCPD-CaCO<sub>3</sub>-based cement was investigated. The reference and composite cements are composed of a nanocrystalline carbonated apatite, similar to bone mineral, and an excess of unreacted vaterite (CaCO<sub>3</sub>). The incorporation of AG increased the composite cement's total porosity compared to the reference cement (CR). The evaluation of the injectability and cohesion properties showed that adding 10 wt % of AG resulted in a total extrusion of the paste with an improvement in the cohesion of the cement paste. The compressive strength of the cements raised from 3.2 for CR up to 7 MPa with the addition of 10 % of AG and Na<sub>2</sub>HPO<sub>4</sub> . The setting time is significantly reduced by introducing Na<sub>2</sub>HPO<sub>4</sub>, resulting in appropriate values (≤ 30 min) for clinical use. Moreover, incorporating 3 wt % of FS in the composite cements had no significant effect on their features. The release study of FS-loaded composites showed sustained and controlled release profiles, with daily released amounts at the therapeutic level. The antibacterial activity of the designed FS-loaded composites demonstrated the effectiveness of the specimens in inhibiting the growth of <em>S. Aureus</em>. Furthermore, the in vitro biological tests did not show any toxicity of the tested cements towards hPBMCs, thereby confirming their biocompatibility.</div></div>","PeriodicalId":47623,"journal":{"name":"Materialia","volume":"38 ","pages":"Article 102248"},"PeriodicalIF":3.0000,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Materialia","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S258915292400245X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MATERIALS SCIENCE, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
Abstract
The present work focuses on the development of composite cements based on dicalcium phosphate dihydrate (DCPD), calcium carbonate CaCO3, sodium alginate (AG), and sodium fusidate (FS). The effect of AG, setting accelerator (0.5 M of Na2HPO4), and antibacterial agent (FS) on the features (setting ability, injectability, cohesion, and compressive strength) of DCPD-CaCO3-based cement was investigated. The reference and composite cements are composed of a nanocrystalline carbonated apatite, similar to bone mineral, and an excess of unreacted vaterite (CaCO3). The incorporation of AG increased the composite cement's total porosity compared to the reference cement (CR). The evaluation of the injectability and cohesion properties showed that adding 10 wt % of AG resulted in a total extrusion of the paste with an improvement in the cohesion of the cement paste. The compressive strength of the cements raised from 3.2 for CR up to 7 MPa with the addition of 10 % of AG and Na2HPO4 . The setting time is significantly reduced by introducing Na2HPO4, resulting in appropriate values (≤ 30 min) for clinical use. Moreover, incorporating 3 wt % of FS in the composite cements had no significant effect on their features. The release study of FS-loaded composites showed sustained and controlled release profiles, with daily released amounts at the therapeutic level. The antibacterial activity of the designed FS-loaded composites demonstrated the effectiveness of the specimens in inhibiting the growth of S. Aureus. Furthermore, the in vitro biological tests did not show any toxicity of the tested cements towards hPBMCs, thereby confirming their biocompatibility.
期刊介绍:
Materialia is a multidisciplinary journal of materials science and engineering that publishes original peer-reviewed research articles. Articles in Materialia advance the understanding of the relationship between processing, structure, property, and function of materials.
Materialia publishes full-length research articles, review articles, and letters (short communications). In addition to receiving direct submissions, Materialia also accepts transfers from Acta Materialia, Inc. partner journals. Materialia offers authors the choice to publish on an open access model (with author fee), or on a subscription model (with no author fee).