Effects of dietary lipid levels on antioxidant capacity, inflammatory response and endoplasmic reticulum stress in immune organ of turbot (Scophthalmus maximus L.)

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2024-10-18 DOI:10.1016/j.aqrep.2024.102434

Dan Xu , Yingming Yang , Junrong Feng , Yongzheng Tang , Pei Yang , Fangrui Lou

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引用次数: 0

Abstract

Lipid, one of the most important macronutrients of organism, can provide energy and essential fatty acids for aquatic animals. Excessive lipid level in diet has negative effects on fish health. However, the regulation mechanism of lipid level on the immunity of marine fish is still not completely understood. Therefore, a 56-day feeding trial was conducted to evaluate the effects of dietary lipid levels on antioxidant capacity, inflammatory response and endoplasmic reticulum stress in immune organs of turbot (Scophthalmus maximus L.). Turbot (9.87 ± 0.13 g) were fed a diet with a low (lipid 6 %, L6), medium (lipid 12 %, L12) and high (lipid 18 %, L18) crude lipid content. Results showed that the L18 group significantly reduced the survival rate during the V. harveyi challenge test compared with L6 and L12 groups. The respiratory burst activity, lysozyme (LZM) and alkaline phosphatase (AKP) in serum were significantly decreased in L6 and L18 groups. Moreover, the L18 group significantly decreased the T-AOC, superoxide dismutase (SOD) and catalase (CAT) activities in head kidney and spleen than that in the L12 group, while the malondialdehyde (MDA) content had no significant changes. Furthermore, the L18 group significantly upregulated the pro-inflammatory genes (tnf-α, il-1β, ifn-γ, p65 and caspase7) expression and the phosphorylation level of the c-Jun N-terminal kinase (JNK) - Mitogen-activated protein kinase (MAPK) pathway in head kidney. Similarly, in spleen, the pro-inflammatory genes (il-1β, ifn-γ, p65 and caspase7) expression were also significantly upregulated in the L18 group. In addition, the endoplasmic reticulum (ER) stress related genes and proteins expression in head kidney and spleen were also significantly upregulated in the L18 group. Overall, these results revealed that the high lipid level in diet suppressed antioxidant capacity and induced inflammatory response of turbot. This study could advance the understanding of effects of dietary lipid levels on turbot (Scophthalmus maximus L.) and improve the utilization rate of high-fat diets in aquaculture.

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膳食脂质水平对大菱鲆免疫器官抗氧化能力、炎症反应和内质网应激的影响

脂质是生物体最重要的宏量营养素之一，可为水生动物提供能量和必需脂肪酸。膳食中脂质含量过高会对鱼类健康产生负面影响。然而，脂质水平对海水鱼免疫力的调节机制仍不完全清楚。因此，本研究进行了一项为期 56 天的喂养试验，以评估日粮脂质水平对大菱鲆（Scophthalmus maximus L.）免疫器官抗氧化能力、炎症反应和内质网应激的影响。给大菱鲆（9.87 ± 0.13 g）喂食低（脂质含量为 6 %，L6）、中（脂质含量为 12 %，L12）和高（脂质含量为 18 %，L18）粗脂含量的日粮。结果表明，与 L6 和 L12 组相比，L18 组在 V. harveyi 挑战试验中的存活率明显降低。L6组和L18组血清中的呼吸爆发活性、溶菌酶（LZM）和碱性磷酸酶（AKP）均显著下降。此外，与 L12 组相比，L18 组头部肾脏和脾脏中的 T-AOC、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性明显降低，而丙二醛（MDA）含量无明显变化。此外，L18 组明显上调头肾中促炎基因（tnf-α、il-1β、ifn-γ、p65 和 caspase7）的表达和 c-Jun N 端激酶（JNK）-丝裂原活化蛋白激酶（MAPK）通路的磷酸化水平。同样，在脾脏中，L18 组促炎基因（il-1β、ifn-γ、p65 和 caspase7）的表达也显著上调。此外，头部肾脏和脾脏中的内质网（ER）应激相关基因和蛋白的表达在 L18 组也明显上调。总之，这些结果表明，高脂饮食抑制了大菱鲆的抗氧化能力，并诱发了炎症反应。该研究有助于进一步了解日粮脂质水平对多宝鱼（Scophthalmus maximus L.）的影响，提高高脂日粮在水产养殖中的利用率。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.