Enhancing growth performance, antioxidant defense, immunity response, and resistance against heat stress in Nile Tilapia (Oreochromis niloticus) fed Saccharomyces boulardii and/or Bifidobacterium bifidum

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2024-10-30 DOI:10.1016/j.aqrep.2024.102462

Nika Mashhadizadeh , Shiva Khezri , Delaram Eslimi Esfahani , Sedigheh Mohammadzadeh , Ehsan Ahmadifar , Mehdi Ahmadifar , Mohsen Shahriari Moghadam , Ehab El-Haroun

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引用次数: 0

Abstract

This study evaluated the benefits of supplementing Nile tilapia diets with probiotics Saccharomyces boulardii (SB) and Bifidobacterium bifidum (BB) in terms of growth, antioxidant status, immune-related genes, and resistance against heat stress. Fish (with an initial weight of 3.85 ± 0.50 g) organized into four triplicate treatment groups defined as control (T0, no supplementation), Saccharomyces boulardii (T1, 10¹⁰ CFU/g), Bifidobacterium bifidum (T2, 1.5×10 8 CFU/ mL) and a mixture of both (T3). This trial continued over a 56-day feeding period and fish were sampled for analysis and thereafter, Nile tilapia was exposed to heat shock under a high temperature of 32°C for 48 hours. Results revealed that the highest final weight (FW), weight gain (WG), and specific growth rate (SGR) as well as significantly lower feed conversion ratio (FCR) were observed in the T3 compared to other groups (P < 0.05). Alternative complement activity (ACH50), total Protein (TP), and lysozyme (LZM) improved in fish-fed supplemented diets, and the highest value was observed in T2 and T3 (P < 0.05). Moreover, this combination enhanced antioxidant status, as evidenced by elevated levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT), reflecting improved defense against oxidative stress (P < 0.05). Gene expression analyses revealed increased expression of immune-related genes such as Interleukin 8, Interleukin10, Interleukin1β, tumor necrosis factor, and LYZ as well as antioxidant genes including CAT, Gpx, glutathione-disulfide reductase, SOD, in test groups compared to the control group (P < 0.05). The survival rate in Nile tilapia after heat stress was significantly higher in T3 compared to other groups (P < 0.05). It was concluded from this experiment that dietary inclusion of the probiotics species in combination could be promote growth and immune responses of O. niloticus.

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提高饲喂布拉氏酵母菌和/或双歧杆菌的尼罗罗非鱼（Oreochromis niloticus）的生长性能、抗氧化防御能力、免疫反应和抗热应激能力

本研究评估了在尼罗罗非鱼日粮中添加布拉氏酵母菌（SB）和双歧杆菌（BB）益生菌对其生长、抗氧化状态、免疫相关基因和抗热应激能力的益处。鱼类（初始体重为 3.85 ± 0.50 克）被分为四个一式三份的处理组，分别为对照组（T0，不添加）、布拉氏酵母菌组（T1，1010 CFU/g）、双歧杆菌组（T2，1.5×10 8 CFU/ mL）和两者混合组（T3）。该试验持续了 56 天的饲养期，并对鱼进行了取样分析，之后，尼罗罗非鱼在 32°C 的高温下受到 48 小时的热休克。结果显示，与其他组相比，T3 组的最终体重（FW）、增重（WG）和特定生长率（SGR）最高，饲料转化率（FCR）明显较低（P < 0.05）。补充日粮的替代补体活性（ACH50）、总蛋白（TP）和溶菌酶（LZM）均有所提高，其中 T2 和 T3 组的数值最高（P < 0.05）。此外，这种组合提高了抗氧化状态，表现为超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和过氧化氢酶（CAT）水平的升高，反映了对氧化应激的防御能力的提高（P <0.05）。基因表达分析表明，与对照组相比，试验组白细胞介素 8、白细胞介素 10、白细胞介素 1β、肿瘤坏死因子和 LYZ 等免疫相关基因以及 CAT、Gpx、谷胱甘肽-二硫化物还原酶、SOD 等抗氧化基因的表达量增加（P <0.05）。与其他组相比，T3 组尼罗罗非鱼在热应激后的存活率明显更高（P < 0.05）。本实验得出的结论是，膳食中混合添加益生菌物种可促进尼罗罗非鱼的生长和免疫反应。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.