Eucommia ulmoides Oliv. leaf extract: Effects on growth, antioxidant capacity, and lipid metabolism in Red swamp crayfish (Procambarus clarkii)

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2024-10-18 DOI:10.1016/j.aqrep.2024.102425

Dan Yang , Wenbo Sun , Mengdan Hou , Chuanbo Xiao , Honghao Jin , Yong Lin , Dapeng Wang , Hua Ye , Hui Luo

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Abstract

The addition of Eucommia ulmoides Oliv. leaf extract (ELE) enhances growth, immunity and antioxidant capacity in crustaceans, which has not been studied on the red swamp crayfish (Procambarus clarkii). Hence, this study aimed to investigate the effect of ELE on the growth, antioxidant capacity, digestive enzymes activities, and lipid metabolism of red swamp crayfish. A total of 240 individuals of red swamp crayfish were randomly assigned to four groups. Each group was fed with feed a diet supplemented with varying doses of ELE: 0 g/kg (CK), 0.5 g/kg (T2), 1.0 g/kg (T3), and 1.5 g/kg (T4). After 30 days, the results indicated that T2 group exhibited a significantly higher final average weight, weight gain rate, and specific growth rate compared to the CK (P<0.05). Significantly lower feed conversion ratios than the CK (P<0.05) were observed in the T2 and T3 groups. For antioxidant indicators, hepatopancreas superoxide dismutase (SOD) activity was observed to significantly increased in the T3 and T4 groups, while levels of malondialdehyde (MDA) in which were significantly reduced in hepatopancreas and hemolymph (P<0.05). Regarding biochemical indicators and intestinal function, significant enhancement of lipase (LPS) activity was observed in the T2 group, which decreased in the T4, compared to the CK (P<0.05). Moreover, significant decrease of the glucose (GLU), total triglyceride (TG), and total cholesterol (TC) content of the hemolymph (P<0.05) were observed in the T4 group. Transcriptome analysis revealed that differentially expressed genes in the T4 group were primarily enriched in peroxisome proliferator-activated receptor (PPAR), and lipid metabolism pathways. Furthermore, it seems that ELE supplements significantly upregulated the expression of apoa-II, acaox1, acox3, me1, and plin1 in the PPAR signaling pathway to regulate lipid metabolism. In conclusion, 0.5 g/kg and 1.5 g/kg ELL addition amount can enhance growth performance, antioxidant capacity, and lipid metabolism of red swamp crayfish.

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杜仲叶提取物：对红沼螯虾（Procambarus clarkii）生长、抗氧化能力和脂质代谢的影响

添加杜仲叶提取物（Eucommia ulmoides Oliv. leaf extract，ELE）可提高甲壳类动物的生长、免疫力和抗氧化能力，但尚未对红色沼泽小龙虾（Procambarus clarkii）进行研究。因此，本研究旨在探讨榄香烯对红沼泽小龙虾的生长、抗氧化能力、消化酶活性和脂质代谢的影响。研究人员将 240 只红色沼泽小龙虾随机分为四组。每组喂食添加不同剂量 ELE 的饲料：0 克/千克（CK）、0.5 克/千克（T2）、1.0 克/千克（T3）和 1.5 克/千克（T4）。30 天后，结果表明 T2 组的最终平均体重、增重率和特定生长率均显著高于 CK 组（P<0.05）。T2 和 T3 组的饲料转化率明显低于 CK 组（P<0.05）。在抗氧化指标方面，观察到肝胰腺超氧化物歧化酶（SOD）活性在 T3 和 T4 组显著增加，而肝胰腺和血淋巴中的丙二醛（MDA）水平显著降低（P<0.05）。在生化指标和肠道功能方面，与 CK 组相比，T2 组的脂肪酶（LPS）活性明显增强，而 T4 组则有所下降（P<0.05）。此外，血淋巴中的葡萄糖（GLU）、总甘油三酯（TG）和总胆固醇（TC）含量在 T4 组明显下降（P<0.05）。转录组分析表明，T4 组的差异表达基因主要集中在过氧化物酶体增殖激活受体（PPAR）和脂质代谢通路。此外，ELE补充剂似乎能显著上调PPAR信号通路中apoa-II、acaox1、acox3、me1和plin1的表达，从而调节脂质代谢。总之，0.5 g/kg 和 1.5 g/kg 的 ELL 添加量可提高红沼泽小龙虾的生长性能、抗氧化能力和脂质代谢。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.