Effect of tropolone on gray mold rot in harvested fruit and its involved mechanism

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2024-10-19 DOI:10.1016/j.postharvbio.2024.113255

Daoguo Chen , Yongxian Chen , Tong Chen , Yong Chen , Yong Xu , Andreas Blennow , Boqiang Li , Shiping Tian

{"title":"Effect of tropolone on gray mold rot in harvested fruit and its involved mechanism","authors":"Daoguo Chen , Yongxian Chen , Tong Chen , Yong Chen , Yong Xu , Andreas Blennow , Boqiang Li , Shiping Tian","doi":"10.1016/j.postharvbio.2024.113255","DOIUrl":null,"url":null,"abstract":"<div><div><em>Botrytis cinerea</em> is one of the most devastating pathogen responsible for postharvest decay of fresh fruits and vegetables. However, traditional fungicide application is inclined to develop resistance strains, complicating the control of gray mold. Hence, there is an urgent need for exploiting new antifungal active substances and enhancing their antifungal activity in an environmentally friendly manner. In this study, tropolone was demonstrated efficacy in inhibiting <em>B. cinerea</em> mycelial growth at relatively low (40 mg L<sup>−1</sup>) tropolone concentrations and spore germination at even lower (10 mg L<sup>−1</sup>) tropolone concentrations. The antifungal mechanisms were linked to the disruption of spore germination-related gene expression rather than directly killing <em>B. cinerea</em> through cell membrane disruption. The inhibition of spore germination hindered the penetration of <em>B. cinerea</em> into host cells, thereby reducing disease severity in harvested apples and strawberries. To increase the protective effect of tropolone, we prepared small-sized maize starch nanoparticles loaded with tropolone and confirmed an enhanced antifungal effect. This study provides a theoretical foundation for the application of small-sized maize starch nanoparticles to increase antifungal effect and reduce fungicide application in postharvest disease control.</div></div>","PeriodicalId":20328,"journal":{"name":"Postharvest Biology and Technology","volume":"219 ","pages":"Article 113255"},"PeriodicalIF":6.4000,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Postharvest Biology and Technology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0925521424005003","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

Botrytis cinerea is one of the most devastating pathogen responsible for postharvest decay of fresh fruits and vegetables. However, traditional fungicide application is inclined to develop resistance strains, complicating the control of gray mold. Hence, there is an urgent need for exploiting new antifungal active substances and enhancing their antifungal activity in an environmentally friendly manner. In this study, tropolone was demonstrated efficacy in inhibiting B. cinerea mycelial growth at relatively low (40 mg L⁻¹) tropolone concentrations and spore germination at even lower (10 mg L⁻¹) tropolone concentrations. The antifungal mechanisms were linked to the disruption of spore germination-related gene expression rather than directly killing B. cinerea through cell membrane disruption. The inhibition of spore germination hindered the penetration of B. cinerea into host cells, thereby reducing disease severity in harvested apples and strawberries. To increase the protective effect of tropolone, we prepared small-sized maize starch nanoparticles loaded with tropolone and confirmed an enhanced antifungal effect. This study provides a theoretical foundation for the application of small-sized maize starch nanoparticles to increase antifungal effect and reduce fungicide application in postharvest disease control.

查看原文本刊更多论文

特罗波隆对采收水果灰霉病的影响及其作用机制

灰霉病菌是造成新鲜水果和蔬菜采后腐烂的最具破坏性的病原体之一。然而，传统的杀真菌剂容易产生抗性菌株，使灰霉病的防治工作变得更加复杂。因此，迫切需要开发新的抗真菌活性物质，并以环境友好的方式提高其抗真菌活性。在这项研究中，在相对较低（40 毫克/升-1）的特罗波隆浓度下，特罗波隆就能有效抑制灰霉病菌菌丝的生长，而在更低（10 毫克/升-1）的特罗波隆浓度下，孢子的萌发也会受到抑制。抗真菌机制与破坏孢子萌发相关基因的表达有关，而不是通过破坏细胞膜直接杀死 B. cinerea。对孢子萌发的抑制阻碍了 B. cinerea 进入寄主细胞，从而降低了收获的苹果和草莓的病害严重程度。为了增强特罗波隆的保护作用，我们制备了负载特罗波隆的小尺寸玉米淀粉纳米颗粒，并证实其抗真菌效果得到了增强。这项研究为应用小型玉米淀粉纳米颗粒提高抗真菌效果和减少采后病害防治中杀菌剂的应用提供了理论基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.