Phlorotannins extracted from Sargassum ilicifolium as a way to control spoilage molds and extend the shelf life of Agaricus bisporus mushrooms

IF 6.4 1区农林科学 Q1 AGRONOMY

Postharvest Biology and Technology Pub Date : 2024-10-18 DOI:10.1016/j.postharvbio.2024.113267

Parinaz Gholami , Sara Chadorshabi , Ehsan Shamloo , Salim Sharifian , Maryam Mahmoudzadeh

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引用次数: 0

Abstract

Phlorotannins (PTs) are compounds with notable bioactive characteristics obtained from brown algae. In the present study, PTs from the Sargassum ilicifolium seaweed were extracted, characterized, and evaluated for their anti-polyphenol oxidase (PPO) enzyme activity and in vitro antifungal activity against spoilage molds (Aspergillus flavus and Penicillium expansum) of button mushrooms. Then, PTs were used as a coating in combination with alginate (AL) to improve the shelf life of Agaricus bisporus mushrooms under storage conditions. HPLC analysis identified phloroglucinol as the predominant component in the PT extract (18.1 %), which demonstrated an 86 ± 1.02 % radical scavenging activity in the DPPH test. The PPO enzyme activity significantly decreased from 320.34 ± 2.1 units to 34.4 ± 1.4 units in the presence of 1.5 mL of PT extract. The PT extract's antifungal properties were observed through well diffusion assays, mycelial growth inhibition, and scanning electron microscopy on treated spoilage mold colonies. Better preservation of the physicochemical properties of mushrooms coated with AL + PT was confirmed through various tests, including assessments of weight loss, browning, malondialdehyde levels, total phenol content, and vitamin C content. Additionally, sensorial tests and evaluations of visual and microstructure changes during storage at 4 °C confirmed the enhanced preservation after treatment. Findings indicated that in addition to the antifungal properties of S. ilicifolium-derived PT, which can act as a natural antifungal, this substance, when used in combination with AL coating, can increase the shelf life of A. bisporus mushrooms 12 d of storage at 4 °C.

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从马尾藻（Sargassum ilicifolium）中提取的叶绿单宁可用于控制腐败霉菌并延长双孢蘑菇的货架期

叶单宁（PTs）是从褐藻中提取的具有显著生物活性特征的化合物。本研究从马尾藻（Sargassum ilicifolium）海藻中提取了PTs，对其进行了表征，并评估了它们的抗多酚氧化酶（PPO）酶活性以及对金针菇腐败霉菌（黄曲霉和扩张青霉）的体外抗真菌活性。然后，将 PTs 与海藻酸盐（AL）结合用作涂层，以提高双孢蘑菇在贮藏条件下的保质期。高效液相色谱分析发现，PT 提取物中的主要成分是氯葡萄糖醇（18.1%），其在 DPPH 试验中的自由基清除活性为 86 ± 1.02%。在 1.5 mL PT 提取物存在的情况下，PPO 酶活性从 320.34 ± 2.1 单位明显降低到 34.4 ± 1.4 单位。通过井扩散试验、抑制菌丝生长和对处理过的腐败霉菌菌落进行扫描电子显微镜观察，可以发现 PT 提取物具有抗真菌特性。通过各种测试，包括对重量损失、褐变、丙二醛水平、总酚含量和维生素 C 含量的评估，证实了涂有 AL + PT 的蘑菇能更好地保持理化特性。此外，感官测试以及对 4 °C 储藏期间视觉和微观结构变化的评估也证实了处理后蘑菇的保存效果得到了增强。研究结果表明，除了 S. ilicifolium 衍生 PT 的抗真菌特性（可作为天然抗真菌剂）外，这种物质与 AL 涂层结合使用时，还能延长双孢蘑菇在 4 °C 下储存 12 天的保质期。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Postharvest Biology and Technology 农林科学-农艺学

CiteScore

12.00

自引率

11.40%

发文量

309

审稿时长

38 days

期刊介绍： The journal is devoted exclusively to the publication of original papers, review articles and frontiers articles on biological and technological postharvest research. This includes the areas of postharvest storage, treatments and underpinning mechanisms, quality evaluation, packaging, handling and distribution of fresh horticultural crops including fruit, vegetables, flowers and nuts, but excluding grains, seeds and forages. Papers reporting novel insights from fundamental and interdisciplinary research will be particularly encouraged. These disciplines include systems biology, bioinformatics, entomology, plant physiology, plant pathology, (bio)chemistry, engineering, modelling, and technologies for nondestructive testing. Manuscripts on fresh food crops that will be further processed after postharvest storage, or on food processes beyond refrigeration, packaging and minimal processing will not be considered.