Crocin has a greater therapeutic role in the restoration of behavioral impairments caused by maternal social isolation in adolescent than in adult offspring probably through GSK-3beta downregulation

IF 1.7 4区心理学 Q3 PSYCHOLOGY, BIOLOGICAL

Learning and Motivation Pub Date : 2024-10-23 DOI:10.1016/j.lmot.2024.102060

Hanie Karimi , Marjan Mohamadian , Paniz Azizi , Pouya Ghasemi , Mokhtar Karimi , Tannaz Layegh , Majid Rahmatkhah-Yazdi , Salar Vaseghi

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引用次数: 0

Abstract

Parental stress drastically impairs cognitive and behavioral functions of the offspring. Social isolation, as one of the most stressful conditions for social animals including rats induces a wide range of destructive effects on behavioral functions. On the other hand, Crocin (active component of Crocus sativa) induces pro-cognitive and neuroprotective effects. In the present study, we aimed to investigate the effects of prolonged maternal social isolation (pre-gestational) and crocin treatment on cognitive and behavioral functions in both adolescent and adult offspring. Female adult rats (mothers) were socially isolated from PND 30 to PND 80 (50 days). The treatment was performed by intraperitoneal injections of crocin (10, 30, and 50 mg/kg) during PND 39–45 or PND 59–65 (7 consecutive days), in the rat offspring. Behavioral assessments were done when the offspring were on PND 45 (adolescent) or PND 65 (adult). The expression level of Glycogen synthase kinase-3 beta (GSK-3beta) in the hippocampus was measured using real-time PCR. The results showed maternal social isolation decreased locomotion and impaired memory in adolescents, and increased anxiety- and depressive-like behaviors and GSK-3beta level, and decreased pain threshold in both adolescents and adults. Crocin (30 and 50 mg/kg) restored or attenuated the effect of maternal social isolation on behavioral functions and GSK-3beta in adolescents and adults, with more effect in adolescents. In conclusion, we showed that crocin treatment can restore the destructive effects of maternal social isolation stress in the offspring, with stronger therapeutic effects in adolescents. Also, GSK-3beta downregulation may underlie the beneficial effects of crocin.

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与成年后代相比，克罗霉素可能通过下调 GSK-3beta 在恢复青少年因母体社会隔离造成的行为障碍方面发挥着更大的治疗作用

父母的压力会严重损害后代的认知和行为功能。社会隔离是包括大鼠在内的社会性动物所面临的最大压力之一，会对行为功能产生广泛的破坏性影响。而 Crocin（茜草的活性成分）则具有促进认知和保护神经的作用。在本研究中，我们旨在探讨母体长期社会隔离（妊娠前）和巴豆苷处理对青少年和成年后代认知和行为功能的影响。雌性成年大鼠（母鼠）在 PND 30 至 PND 80（50 天）期间被社会隔离。在PND 39-45或PND 59-65（连续7天）期间，对后代大鼠腹腔注射黄曲霉素（10、30和50毫克/千克）。后代在 PND 45（青春期）或 PND 65（成年期）时进行行为评估。海马中糖原合成酶激酶-3 beta（GSK-3beta）的表达水平采用实时 PCR 法进行测量。结果表明，母性社会隔离会降低青少年的运动能力并损害其记忆力，同时会增加青少年和成人的焦虑和抑郁样行为及 GSK-3beta 水平，并降低疼痛阈值。藏霉素（30和50毫克/千克）可恢复或减轻母体社会隔离对青少年和成人行为功能和GSK-3beta的影响，其中对青少年的影响更大。总之，我们的研究表明，羊角霉素治疗可以恢复母体社会隔离应激对后代的破坏作用，对青少年的治疗效果更强。此外，GSK-3beta的下调可能是黄花苷有益作用的基础。

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来源期刊

Learning and Motivation Multiple-

CiteScore

2.90

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0.00%

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期刊介绍： Learning and Motivation features original experimental research devoted to the analysis of basic phenomena and mechanisms of learning, memory, and motivation. These studies, involving either animal or human subjects, examine behavioral, biological, and evolutionary influences on the learning and motivation processes, and often report on an integrated series of experiments that advance knowledge in this field. Theoretical papers and shorter reports are also considered.