Metagenomics reveals soil nitrogen cycling after vegetation restoration: Influence of different vegetation restoration strategies

IF 4.8 2区农林科学 Q1 SOIL SCIENCE

Applied Soil Ecology Pub Date : 2024-10-22 DOI:10.1016/j.apsoil.2024.105695

Hengkang Xu , Chao Chen , Wenqing Chen , Zhuo Pang , Guofang Zhang , Weiwei Zhang , Haiming Kan

{"title":"Metagenomics reveals soil nitrogen cycling after vegetation restoration: Influence of different vegetation restoration strategies","authors":"Hengkang Xu , Chao Chen , Wenqing Chen , Zhuo Pang , Guofang Zhang , Weiwei Zhang , Haiming Kan","doi":"10.1016/j.apsoil.2024.105695","DOIUrl":null,"url":null,"abstract":"<div><div>Microorganisms play a crucial role in the cycling and transformation of nitrogen (N) within ecosystems. However, there is limited understanding regarding the impact of vegetation restoration on soil N cycling. A field study investigated the effects of different vegetation restoration strategies on soil microbial N cycling in sandy deserts of northern China including the use of metagenomic sequencing technology. The restoration strategies included the planting of Bromus inermis Leyss (SB), Medicago sativa L. (AF), and combined planting of Salix babylonica L. and Bromus inermis Leyss (FG). Compared with the natural restoration (CK), the abundance of genes related to soil N nitrification and denitrification processes was found to be higher in the AF and SB restoration strategies. On the other hand, the SB strategy specifically led to an enrichment of genes linked to dissimilatory nitrate reduction to ammonium (DNRA). Compared with the CK, the abundance of amoC/pmoC, hao and nxrA involved in soil N nitrification were higher in AF. The diversity of the fungal community was more strongly influenced by various vegetation restoration strategies compared to bacteria. Interestingly, FG had no significant effect on bacterial and fungal diversity compared to CK. However, alpha diversity of fungal communities was lower in AF and higher in SB compared to the CK. Soil pH was positively related to functional genes that drive nitrification and denitrification processes (nirK, amoC/pmoC, and hao). N fixation and DNRA exhibited a negative correlation with both microbial biomass carbon and microbial biomass nitrogen. Consequently, the planting of AF and SB hold significant importance in promoting soil N cycling within degraded lands. The study offered valuable insights into the microbial functional potentials associated with long-term vegetation restoration efforts, potentially bearing significant implications for soil N cycling in the degraded lands of northern China.</div></div>","PeriodicalId":8099,"journal":{"name":"Applied Soil Ecology","volume":"204 ","pages":"Article 105695"},"PeriodicalIF":4.8000,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Soil Ecology","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0929139324004268","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SOIL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

Microorganisms play a crucial role in the cycling and transformation of nitrogen (N) within ecosystems. However, there is limited understanding regarding the impact of vegetation restoration on soil N cycling. A field study investigated the effects of different vegetation restoration strategies on soil microbial N cycling in sandy deserts of northern China including the use of metagenomic sequencing technology. The restoration strategies included the planting of Bromus inermis Leyss (SB), Medicago sativa L. (AF), and combined planting of Salix babylonica L. and Bromus inermis Leyss (FG). Compared with the natural restoration (CK), the abundance of genes related to soil N nitrification and denitrification processes was found to be higher in the AF and SB restoration strategies. On the other hand, the SB strategy specifically led to an enrichment of genes linked to dissimilatory nitrate reduction to ammonium (DNRA). Compared with the CK, the abundance of amoC/pmoC, hao and nxrA involved in soil N nitrification were higher in AF. The diversity of the fungal community was more strongly influenced by various vegetation restoration strategies compared to bacteria. Interestingly, FG had no significant effect on bacterial and fungal diversity compared to CK. However, alpha diversity of fungal communities was lower in AF and higher in SB compared to the CK. Soil pH was positively related to functional genes that drive nitrification and denitrification processes (nirK, amoC/pmoC, and hao). N fixation and DNRA exhibited a negative correlation with both microbial biomass carbon and microbial biomass nitrogen. Consequently, the planting of AF and SB hold significant importance in promoting soil N cycling within degraded lands. The study offered valuable insights into the microbial functional potentials associated with long-term vegetation restoration efforts, potentially bearing significant implications for soil N cycling in the degraded lands of northern China.

Abstract Image

查看原文本刊更多论文

元基因组学揭示植被恢复后的土壤氮循环：不同植被恢复策略的影响

微生物在生态系统内氮（N）的循环和转化过程中发挥着至关重要的作用。然而，人们对植被恢复对土壤氮循环的影响了解有限。一项实地研究调查了不同植被恢复策略对中国北方沙质沙漠土壤微生物氮循环的影响，包括使用元基因组测序技术。植被恢复策略包括种植 Bromus inermis Leyss（SB）、Medicago sativa L.（AF）以及 Salix babylonica L.和 Bromus inermis Leyss（FG）的联合种植。与自然恢复（CK）相比，AF 和 SB 恢复策略中与土壤氮硝化和反硝化过程相关的基因丰度较高。另一方面，SB 策略特别导致了与硝酸盐异氨还原（DNRA）相关基因的丰富。与 CK 相比，AF 中参与土壤氮硝化的 amoC/pmoC、hao 和 nxrA 的丰度更高。与细菌相比，各种植被恢复策略对真菌群落多样性的影响更大。有趣的是，与 CK 相比，FG 对细菌和真菌多样性没有显著影响。然而，与 CK 相比，AF 真菌群落的 alpha 多样性较低，而 SB 真菌群落的 alpha 多样性较高。土壤 pH 值与驱动硝化和反硝化过程的功能基因（nirK、amoC/pmoC 和 hao）呈正相关。固氮和 DNRA 与微生物生物量碳和微生物生物量氮均呈负相关。因此，种植 AF 和 SB 对促进退化土地的土壤氮循环具有重要意义。该研究为了解与长期植被恢复工作相关的微生物功能潜力提供了宝贵的见解，可能对中国北方退化土地的土壤氮循环具有重要意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Applied Soil Ecology 农林科学-土壤科学

CiteScore

9.70

自引率

4.20%

发文量

363

审稿时长

5.3 months

期刊介绍： Applied Soil Ecology addresses the role of soil organisms and their interactions in relation to: sustainability and productivity, nutrient cycling and other soil processes, the maintenance of soil functions, the impact of human activities on soil ecosystems and bio(techno)logical control of soil-inhabiting pests, diseases and weeds.