Massilia shenzhen sp. nov., isolated from blood of one premature infant, causing sepsis

IF 2.1 4区医学 Q3 INFECTIOUS DISEASES

Diagnostic microbiology and infectious disease Pub Date : 2024-10-18 DOI:10.1016/j.diagmicrobio.2024.116566

Xinying Li , Wenxuan Zheng , Tongyu Hao , Ting Yang , Xiaojuan Gao , Xiuming Zhang

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Abstract

This study explores a premature infant with respiratory failure and pneumonia, suggestive of neonatal sepsis. Despite initially negative clinical specimens, blood testing revealed a pathogen. MALDI-TOF-MS and physiological tests initially failed to identify it accurately. Subsequent analysis of the 16S rRNA gene, housekeeping genes, and whole genome sequencing placed it in the genus Massilia. Average Nucleotide Identities (ANIs) indicated 88.47 % similarity with the type strain of Massilia norwichensis. Detailed characterization showed it as Gram-negative, aerobic, flagellated, measuring 0.45–0.55 × 1.75–2.40 μm. Major fatty acids included C16:0, C16:1ω7c, C18:1ω7c, and cyclo-C17:0. Minimum inhibitory concentrations to ceftazidime, penicillin, and meropenem were <0.032 μg/mL, ≤0.75 μg/mL, and <0.002 μg/mL respectively. Phylogenetic analysis, fatty acid composition, and physiological parameters confirmed it as Massilia shenzhen sp. nov., with strain GZ0329^T. Given limited research on Massilia drug resistance, ceftazidime and imipenem show promise in treating Massilia infections.

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从一名早产儿血液中分离出的导致败血症的深圳麻风杆菌新种

本研究探讨了一名早产儿因呼吸衰竭和肺炎而引发的新生儿败血症。尽管临床标本最初呈阴性，但血液检测发现了一种病原体。最初，MALDI-TOF-MS 和生理检测未能准确识别病原体。随后对 16S rRNA 基因、看家基因和全基因组测序进行分析，将其归入 Massilia 属。平均核苷酸同一性（ANIs）表明，它与诺维琴氏马氏菌（Massilia norwichensis）的模式菌株有 88.47 % 的相似性。详细特征显示，它为革兰氏阴性、需氧、鞭毛，大小为 0.45-0.55 × 1.75-2.40 μm。主要脂肪酸包括 C16:0、C16:1ω7c、C18:1ω7c 和环-C17:0。对头孢他啶、青霉素和美罗培南的最小抑菌浓度分别为<0.032 μg/mL、≤0.75 μg/mL和<0.002 μg/mL。通过系统进化分析、脂肪酸组成和生理参数，确认其为深圳麻蝇新种，菌株为 GZ0329T。鉴于对马氏鳃蝇耐药性的研究有限，头孢他啶和亚胺培南有望用于治疗马氏鳃蝇感染。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Diagnostic microbiology and infectious disease 医学-传染病学

CiteScore

5.30

自引率

3.40%

发文量

149

审稿时长

56 days

期刊介绍： Diagnostic Microbiology and Infectious Disease keeps you informed of the latest developments in clinical microbiology and the diagnosis and treatment of infectious diseases. Packed with rigorously peer-reviewed articles and studies in bacteriology, immunology, immunoserology, infectious diseases, mycology, parasitology, and virology, the journal examines new procedures, unusual cases, controversial issues, and important new literature. Diagnostic Microbiology and Infectious Disease distinguished independent editorial board, consisting of experts from many medical specialties, ensures you extensive and authoritative coverage.